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PIE-1 promotes SUMOylation and activation of HDAC1 during the C. elegans oogenesis

Heesun Kim, Yue-He Ding, Shan Lu, Mei-Qing Zuo, Darryl Conte Jr., Meng-Qiu Dong, Craig C. Mello
doi: https://doi.org/10.1101/2020.08.17.253955
Heesun Kim
1RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
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Yue-He Ding
1RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
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Shan Lu
2National Institute of Biological Sciences, Beijing, Beijing 102206, China
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Mei-Qing Zuo
2National Institute of Biological Sciences, Beijing, Beijing 102206, China
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Darryl Conte Jr.
1RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
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Meng-Qiu Dong
2National Institute of Biological Sciences, Beijing, Beijing 102206, China
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Craig C. Mello
1RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA
4Howard Hughes Medical Institute
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  • For correspondence: Craig.Mello@umassmed.edu
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Summary

Germlines shape and balance heredity, integrating and regulating information from both parental and foreign sources. Insights into how the germline handles information have come from the identification of factors that specify or maintain the germline fate. In early C. elegans embryos, the CCCH zinc-finger protein PIE-1 localizes to the germline where it prevents somatic differentiation programs. Here we show that PIE-1 also functions in the meiotic ovary where it becomes SUMOylated and engages the SUMO-conjugating machinery. Using whole-proteome mass spectrometry to detect SUMO-conjugated proteins, we identify HDAC SUMOylation as a target of PIE-1. Our findings suggest that SUMOylation activates HDAC, lowering histone acetylation and enhancing Argonaute-mediated surveillance in the germline.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Supplemental files (table3, 4, and 5) updated. Manuscript revised based on the updated supplemental files. References revised in the manuscript.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 19, 2020.
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PIE-1 promotes SUMOylation and activation of HDAC1 during the C. elegans oogenesis
Heesun Kim, Yue-He Ding, Shan Lu, Mei-Qing Zuo, Darryl Conte Jr., Meng-Qiu Dong, Craig C. Mello
bioRxiv 2020.08.17.253955; doi: https://doi.org/10.1101/2020.08.17.253955
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PIE-1 promotes SUMOylation and activation of HDAC1 during the C. elegans oogenesis
Heesun Kim, Yue-He Ding, Shan Lu, Mei-Qing Zuo, Darryl Conte Jr., Meng-Qiu Dong, Craig C. Mello
bioRxiv 2020.08.17.253955; doi: https://doi.org/10.1101/2020.08.17.253955

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