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Mass Spectrometry Imaging of N-Glycans Reveals Racial Discrepancies in Low Grade Prostate Tumors

Lindsey R. Conroy, Lyndsay E.A. Young, Alexandra E. Stanback, Grant L. Austin, Jinpeng Liu, Jinze Liu, Derek B. Allison, Ramon C. Sun
doi: https://doi.org/10.1101/2020.08.20.260026
Lindsey R. Conroy
1Department of Neuroscience, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
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Lyndsay E.A. Young
2Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
3Markey Cancer Center, Lexington, KY 40536, USA
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Alexandra E. Stanback
2Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
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Grant L. Austin
2Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
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Jinpeng Liu
3Markey Cancer Center, Lexington, KY 40536, USA
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Jinze Liu
4Department of Computer Science, College of Engineering, University of Kentucky, Lexington, KY 40536, USA
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Derek B. Allison
3Markey Cancer Center, Lexington, KY 40536, USA
5Department of Pathology and Laboratory Medicine, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
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Ramon C. Sun
1Department of Neuroscience, College of Medicine, University of Kentucky, Lexington, KY 40536, USA
3Markey Cancer Center, Lexington, KY 40536, USA
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  • For correspondence: ramon.sun@uky.edu
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Abstract

Prostate cancer is the most common cancer in men worldwide. Despite its prevalence, there is a critical knowledge gap regarding the underlining molecular events that result in higher incidence and mortality rate in Black men. Identifying molecular features that separate racial disparities is a critical step in prostate cancer research that could lead to predictive biomarkers and personalized therapy. N-linked glycosylation is a co-translational event during protein folding that modulates a myriad of cellular processes. Recently, aberrant N-linked glycosylation has been reported in prostate cancers. However, the full clinical implications of dysregulated glycosylation in prostate cancer has yet to be explored. Herein, we performed high-throughput matrix-assisted laser desorption ionization mass spectrometry analysis to characterize the N-glycan profile from tissue microarrays of over 100 patient tumors with over 10 years of follow up data. We identified several species of N-glycans that were profoundly different between low grade prostate tumors resected from White and Black patients. Further, these glycans predict opposing overall survival between White and Black patients with prostate cancer. These data suggest differential N-linked glycosylation underline the racial disparity of prostate cancer prognosis. Our study highlights the potential applications of MALDI-MSI for digital pathology and biomarker to study racial disparity of prostate cancer patients.

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Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • The authors have declared that no conflict of interest exists.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted August 22, 2020.
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Mass Spectrometry Imaging of N-Glycans Reveals Racial Discrepancies in Low Grade Prostate Tumors
Lindsey R. Conroy, Lyndsay E.A. Young, Alexandra E. Stanback, Grant L. Austin, Jinpeng Liu, Jinze Liu, Derek B. Allison, Ramon C. Sun
bioRxiv 2020.08.20.260026; doi: https://doi.org/10.1101/2020.08.20.260026
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Mass Spectrometry Imaging of N-Glycans Reveals Racial Discrepancies in Low Grade Prostate Tumors
Lindsey R. Conroy, Lyndsay E.A. Young, Alexandra E. Stanback, Grant L. Austin, Jinpeng Liu, Jinze Liu, Derek B. Allison, Ramon C. Sun
bioRxiv 2020.08.20.260026; doi: https://doi.org/10.1101/2020.08.20.260026

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