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Intra-exon motif correlations as a proxy measure for mean per-tile sequence quality data in RNA-Seq

View ORCID ProfileJamie J. Alnasir, View ORCID ProfileHugh P. Shanahan
doi: https://doi.org/10.1101/2020.08.23.262055
Jamie J. Alnasir
1Department of Computing, Imperial College, London, UK,
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  • For correspondence: j.alnasir@imperial.ac.uk j.alnasir@imperial.ac.uk
Hugh P. Shanahan
2Department of Computer Science, Royal Holloway, Egham, UK
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Abstract

Given the wide variability in the quality of NGS data submitted to public repositories, it is essential to identify methods that can perform quality control on these datasets when additional quality control data, such as mean tile data, is missing. This is particularly important because such datasets are routinely deposited in public archives that now store data at an unprecedented scale. In this paper, we show that correlating counts of reads corresponding to pairs of motifs separated over specific distances on individual exons corresponds to mean tile data in the datasets we analysed, and can therefore be used when mean tile data is not available.

As test datasets we use the H. sapiens IVT (in-vitro transcribed) dataset of Lahens et al., and a D. melanogaster dataset comprising wild and mutant types from Aerts et al.

The intra-exon motif correlations as a function of both GC content parameters are much higher in the IVT-Plasmids mRNA selection free RNA-Seq sample (control) than in the other RNA-Seq samples that did undergo mRNA selection: both ribosomal depletion (IVT-Only) and PolyA selection (IVT-polyA, wild-type, and mutant). There is considerable degradation of similar correlations in the mutant samples from the D. melanogaster dataset. This matches with the available mean tile data that has been gathered for these datasets. We observe that extremely low correlations are indicative of bias of technical origin, such as flowcell errors.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://github.com/jamie-alnasir/hercules

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 24, 2020.
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Intra-exon motif correlations as a proxy measure for mean per-tile sequence quality data in RNA-Seq
Jamie J. Alnasir, Hugh P. Shanahan
bioRxiv 2020.08.23.262055; doi: https://doi.org/10.1101/2020.08.23.262055
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Intra-exon motif correlations as a proxy measure for mean per-tile sequence quality data in RNA-Seq
Jamie J. Alnasir, Hugh P. Shanahan
bioRxiv 2020.08.23.262055; doi: https://doi.org/10.1101/2020.08.23.262055

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