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Meta-azotomics of engineered wastewater treatment processes reveals differential contributions of established and novel models of N-cycling

Mee-Rye Park, Medini K. Annavajhala, Kartik Chandran
doi: https://doi.org/10.1101/2020.08.25.229054
Mee-Rye Park
1Department of Earth and Environmental Engineering, Columbia University, New York, NY 10027, USA
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Medini K. Annavajhala
1Department of Earth and Environmental Engineering, Columbia University, New York, NY 10027, USA
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Kartik Chandran
1Department of Earth and Environmental Engineering, Columbia University, New York, NY 10027, USA
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  • For correspondence: kc2288@columbia.edu
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Abstract

The application of metagenomics and metatranscriptomics to field-scale engineered biological nitrogen removal (BNR) processes revealed a complex N-cycle network (the meta-azotome) therein in terms of microbial structure, potential and extant function. Autotrophic nitrification bore the imprint of well-documented Nitrosomonas and Nitrospira in most systems. However, in select BNR processes, complete ammonia oxidizing bacteria, comammox Nitrospira, unexpectedly contributed more substantially to ammonia oxidation than canonical ammonia oxidizing bacteria, based on metatranscriptomic profiling. Methylotrophic denitrification was distinctly active in methanol-fed reactors but not in glycerol-fed reactors. Interestingly, glycerol metabolism and N-reduction transcript signatures were uncoupled, possibly suggesting the role of other carbon sources in denitrification emanating from glycerol itself or from upstream process reactors. In sum, the meta-azotome of engineered BNR processes revealed both traditional and novel mechanisms of N-cycling. Similar interrogation approaches could potentially inform better design and optimization of wastewater treatment and engineered bioprocesses in general.

Competing Interest Statement

The authors have declared no competing interest.

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Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 25, 2020.
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Meta-azotomics of engineered wastewater treatment processes reveals differential contributions of established and novel models of N-cycling
Mee-Rye Park, Medini K. Annavajhala, Kartik Chandran
bioRxiv 2020.08.25.229054; doi: https://doi.org/10.1101/2020.08.25.229054
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Meta-azotomics of engineered wastewater treatment processes reveals differential contributions of established and novel models of N-cycling
Mee-Rye Park, Medini K. Annavajhala, Kartik Chandran
bioRxiv 2020.08.25.229054; doi: https://doi.org/10.1101/2020.08.25.229054

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