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Loud noise exposure differentially affects subpopulations of auditory cortex pyramidal cells

Ingrid Nogueira, Jessica Winne, Thiago Z. Lima, Thawann Malfatti, Richardson N. Leao, View ORCID ProfileKatarina E. Leao
doi: https://doi.org/10.1101/2020.08.25.264200
Ingrid Nogueira
1Hearing and neuronal activity lab, Brain Institute, Federal University of Rio Grande do Norte, Av. Nascimento de Castro 2155, 59056-450, Natal/RN, Brazil
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Jessica Winne
2Neurodynamics lab, Brain Institute, Federal University of Rio Grande do Norte, Av. Nascimento de Castro 2155, 59056-450, Natal/RN, Brazil
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Thiago Z. Lima
1Hearing and neuronal activity lab, Brain Institute, Federal University of Rio Grande do Norte, Av. Nascimento de Castro 2155, 59056-450, Natal/RN, Brazil
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Thawann Malfatti
1Hearing and neuronal activity lab, Brain Institute, Federal University of Rio Grande do Norte, Av. Nascimento de Castro 2155, 59056-450, Natal/RN, Brazil
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Richardson N. Leao
2Neurodynamics lab, Brain Institute, Federal University of Rio Grande do Norte, Av. Nascimento de Castro 2155, 59056-450, Natal/RN, Brazil
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Katarina E. Leao
1Hearing and neuronal activity lab, Brain Institute, Federal University of Rio Grande do Norte, Av. Nascimento de Castro 2155, 59056-450, Natal/RN, Brazil
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  • ORCID record for Katarina E. Leao
  • For correspondence: katarina.leao@neuro.ufrn.br
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ABSTRACT

Loud noise-exposure generates tinnitus in both humans and animals. Macroscopic studies show that noise exposure affects the auditory cortex; however, cellular mechanisms of tinnitus generation are unclear. Here we compare membrane properties of layer 5 (L5) pyramidal cells (PCs) of the primary auditory cortex (A1) from control and noise-exposed mice. PCs were previously classified in type A or type B based on connectivity and firing properties. Our analysis based on a logistic regression model predicted that afterhyperpolatization and afterdepolarization following the injection of inward and outward current are enough to predict cell type and these features are preserved after noise trauma. One week after a noise-exposure (4-18kHz, 90dB, 1.5 hr, followed by 1.5hr silence) no passive membrane properties of type A or B PCs were altered but principal component analysis showed greater separation between control/noise-exposure recordings for type A neurons. When comparing individual firing properties, noise exposure differentially affected type A and B PC firing frequency in response to depolarizing current steps. Specifically, type A PCs decreased both initial and steady state firing frequency and type B PCs significantly increased steady state firing frequency following noise exposure. These results show that loud noise can cause distinct effects on type A and B L5 auditory cortex PCs one week following noise exposure. As the type A PC electrophysiological profile is correlated to corticofugal L5 neurons, and type B PCs correlate to contralateral projecting PCs these alterations could partially explain the reorganization of the auditory cortex observed in tinnitus patients.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Funding sources: This work is supported by the American Tinnitus Association and the Brazilian National Council for Scientific and Technological Development

  • Conflict of interest: The authors declare no conflict of interest

  • Abbreviations: ADP, afterdepolarization; AHP, afterhyperpolarization; dBSPL, decibel sound pressure level; f-I, frequency over current; L, cortical layer; MANOVA, multivariate analysis of variance; PC, pyramidal cell; PrC, principal component

  • the description of the grin lens/prism probe was incorect. Now the correct specs were added to the methods

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 31, 2020.
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Loud noise exposure differentially affects subpopulations of auditory cortex pyramidal cells
Ingrid Nogueira, Jessica Winne, Thiago Z. Lima, Thawann Malfatti, Richardson N. Leao, Katarina E. Leao
bioRxiv 2020.08.25.264200; doi: https://doi.org/10.1101/2020.08.25.264200
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Loud noise exposure differentially affects subpopulations of auditory cortex pyramidal cells
Ingrid Nogueira, Jessica Winne, Thiago Z. Lima, Thawann Malfatti, Richardson N. Leao, Katarina E. Leao
bioRxiv 2020.08.25.264200; doi: https://doi.org/10.1101/2020.08.25.264200

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