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Single nucleus analysis of Arabidopsis seeds reveals new cell types and imprinting dynamics

View ORCID ProfileColette L. Picard, View ORCID ProfileRebecca A. Povilus, View ORCID ProfileBen P. Williams, View ORCID ProfileMary Gehring
doi: https://doi.org/10.1101/2020.08.25.267476
Colette L. Picard
1Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, USA
2Computational and Systems Biology Graduate Program, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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Rebecca A. Povilus
1Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, USA
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Ben P. Williams
1Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, USA
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Mary Gehring
1Whitehead Institute for Biomedical Research, Cambridge, MA, 02142, USA
3Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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  • For correspondence: mgehring@wi.mit.edu
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Abstract

Seeds are the basis of agriculture, yet their full transcriptional complexity has remained unknown. Here, we employ single-nucleus RNA-sequencing to characterize developing Arabidopsis thaliana seeds, with a focus on endosperm. Endosperm, the site of gene imprinting in plants, mediates the relationship between the maternal parent and embryo. We identify new cell types in the chalazal endosperm region, which interfaces with maternal tissue for nutrient unloading. We further demonstrate that the extent of parental bias of maternally expressed imprinted genes varies with cell cycle phase, and that imprinting of paternally expressed imprinted genes is strongest in chalazal endosperm. These data indicate imprinting in endosperm is heterogeneous and suggest that parental conflict, which is proposed to drive the evolution of imprinting, is fiercest at the boundary between filial and maternal tissues.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 25, 2020.
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Single nucleus analysis of Arabidopsis seeds reveals new cell types and imprinting dynamics
Colette L. Picard, Rebecca A. Povilus, Ben P. Williams, Mary Gehring
bioRxiv 2020.08.25.267476; doi: https://doi.org/10.1101/2020.08.25.267476
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Single nucleus analysis of Arabidopsis seeds reveals new cell types and imprinting dynamics
Colette L. Picard, Rebecca A. Povilus, Ben P. Williams, Mary Gehring
bioRxiv 2020.08.25.267476; doi: https://doi.org/10.1101/2020.08.25.267476

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