Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Base Pairing and Functional Insights into N3-methylcytidine (m3C) in RNA

Song Mao, Phensinee Haruehanroengra, Srivathsan V. Ranganathan, Fusheng Shen, Thomas J. Begley, Jia Sheng
doi: https://doi.org/10.1101/2020.08.27.270322
Song Mao
1Department of Chemistry, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
2The RNA Institute, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Phensinee Haruehanroengra
1Department of Chemistry, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
2The RNA Institute, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Srivathsan V. Ranganathan
2The RNA Institute, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fusheng Shen
1Department of Chemistry, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
2The RNA Institute, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas J. Begley
2The RNA Institute, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
3Department of Biological Science, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jia Sheng
1Department of Chemistry, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
2The RNA Institute, University at Albany, State University of New York, 1400 Washington Ave. Albany, NY, 12222, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: jsheng@albany.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

N3-methylcytidine (m3C) is present in both eukaryotic tRNA and mRNA and plays critical roles in many biological processes. We report the synthesis of the m3C phosphoramidite building block and its containing RNA oligonucleotides. The base-pairing stability and specificity studies show that the m3C modification significantly disrupts the stability of the Watson-Crick C:G pair. Further m3C decreases the base pairing discrimination between C:G and the other mismatched C:A, C:U, and C:C pairs. Our molecular dynamic simulation study further reveals the detailed structural insights into the m3C:G base pairing pattern in an RNA duplex. More importantly, the biochemical investigation of m3C using reverse transcription shows that N3-methylation specifies the C:A pair and induces a G to A mutation using HIV-1-RT, MMLV-RT and MutiScribe™-RT enzymes, all with relatively low replication fidelity. For other reverse transcriptases with higher fidelity like AMV-RT, the methylation could completely shut down DNA synthesis.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted August 28, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Base Pairing and Functional Insights into N3-methylcytidine (m3C) in RNA
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Base Pairing and Functional Insights into N3-methylcytidine (m3C) in RNA
Song Mao, Phensinee Haruehanroengra, Srivathsan V. Ranganathan, Fusheng Shen, Thomas J. Begley, Jia Sheng
bioRxiv 2020.08.27.270322; doi: https://doi.org/10.1101/2020.08.27.270322
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Base Pairing and Functional Insights into N3-methylcytidine (m3C) in RNA
Song Mao, Phensinee Haruehanroengra, Srivathsan V. Ranganathan, Fusheng Shen, Thomas J. Begley, Jia Sheng
bioRxiv 2020.08.27.270322; doi: https://doi.org/10.1101/2020.08.27.270322

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biochemistry
Subject Areas
All Articles
  • Animal Behavior and Cognition (4087)
  • Biochemistry (8762)
  • Bioengineering (6479)
  • Bioinformatics (23341)
  • Biophysics (11750)
  • Cancer Biology (9149)
  • Cell Biology (13248)
  • Clinical Trials (138)
  • Developmental Biology (7417)
  • Ecology (11369)
  • Epidemiology (2066)
  • Evolutionary Biology (15087)
  • Genetics (10399)
  • Genomics (14009)
  • Immunology (9121)
  • Microbiology (22040)
  • Molecular Biology (8779)
  • Neuroscience (47368)
  • Paleontology (350)
  • Pathology (1420)
  • Pharmacology and Toxicology (2482)
  • Physiology (3704)
  • Plant Biology (8050)
  • Scientific Communication and Education (1431)
  • Synthetic Biology (2208)
  • Systems Biology (6016)
  • Zoology (1249)