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Coformulation with tattoo ink for immunological assessment of vaccine immunogenicity in the draining lymph node

Isaac M Barber-Axthelm, Hannah G Kelly, Robyn Esterbauer, Kathleen Wragg, Anne Gibbon, Wen Shi Lee, Adam K Wheatley, Stephen J Kent, Hyon-Xhi Tan, View ORCID ProfileJennifer A Juno
doi: https://doi.org/10.1101/2020.08.27.270975
Isaac M Barber-Axthelm
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
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Hannah G Kelly
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
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Robyn Esterbauer
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
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Kathleen Wragg
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
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Anne Gibbon
2Monash Animal Research Platform, Monash University, Clayton, Victoria 3800, Australia
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Wen Shi Lee
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
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Adam K Wheatley
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
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Stephen J Kent
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
3Australian Research Council Centre for Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, Melbourne, Victoria 3010, Australia
4Melbourne Sexual Health Centre and Department of Infectious Diseases, Alfred Hospital and Central Clinical School, Monash University, Melbourne, Victoria 3004, Australia
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Hyon-Xhi Tan
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
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  • For correspondence: jennifer.juno@unimelb.edu.au hyon.tan@unimelb.edu.au
Jennifer A Juno
1Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne Victoria 3000, Australia
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  • ORCID record for Jennifer A Juno
  • For correspondence: jennifer.juno@unimelb.edu.au hyon.tan@unimelb.edu.au
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Abstract

Characterisation of germinal centre B and T cell responses yields critical insights into vaccine immunogenicity. Non-human primates are a key pre-clinical animal model for human vaccine development, allowing both lymph node and circulating immune responses to be longitudinally sampled for correlates of vaccine efficacy. However, patterns of vaccine antigen drainage via the lymphatics after intramuscular immunisation can be stochastic, driving uneven deposition between lymphoid sites, and between individual lymph nodes within larger clusters. In order to improve the accurate isolation of antigen-exposed lymph nodes during biopsies and necropsies, we developed and validated a method for co-formulating candidate vaccines with tattoo ink, which allows for direct visual identification of vaccine-draining lymph nodes and evaluation of relevant antigen-specific B and T cell responses by flow cytometry. This approach improves the assessment of vaccine-induced immunity in highly relevant non-human primate models.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted August 29, 2020.
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Coformulation with tattoo ink for immunological assessment of vaccine immunogenicity in the draining lymph node
Isaac M Barber-Axthelm, Hannah G Kelly, Robyn Esterbauer, Kathleen Wragg, Anne Gibbon, Wen Shi Lee, Adam K Wheatley, Stephen J Kent, Hyon-Xhi Tan, Jennifer A Juno
bioRxiv 2020.08.27.270975; doi: https://doi.org/10.1101/2020.08.27.270975
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Coformulation with tattoo ink for immunological assessment of vaccine immunogenicity in the draining lymph node
Isaac M Barber-Axthelm, Hannah G Kelly, Robyn Esterbauer, Kathleen Wragg, Anne Gibbon, Wen Shi Lee, Adam K Wheatley, Stephen J Kent, Hyon-Xhi Tan, Jennifer A Juno
bioRxiv 2020.08.27.270975; doi: https://doi.org/10.1101/2020.08.27.270975

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