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Endoderm Nitric Oxide Signals to Regulate Nascent Development of Cardiac Progenitors in Chicken Embryos

Devan H. Shah, Sujoy K. Biswas, Adrian M. Martin, Simone Bianco, Wilfred F. Denetclaw
doi: https://doi.org/10.1101/2020.08.29.272989
Devan H. Shah
1Department of Biology, San Francisco State University, San Francisco, USA
3Center for Cellular Construction, San Francisco Bay Area, USA
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Sujoy K. Biswas
2Department of Industrial and Applied Genomics, AI and Cognitive Software Organization, IBM Almaden Research Center, San Jose, USA
3Center for Cellular Construction, San Francisco Bay Area, USA
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Adrian M. Martin
1Department of Biology, San Francisco State University, San Francisco, USA
3Center for Cellular Construction, San Francisco Bay Area, USA
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Simone Bianco
2Department of Industrial and Applied Genomics, AI and Cognitive Software Organization, IBM Almaden Research Center, San Jose, USA
3Center for Cellular Construction, San Francisco Bay Area, USA
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Wilfred F. Denetclaw
1Department of Biology, San Francisco State University, San Francisco, USA
3Center for Cellular Construction, San Francisco Bay Area, USA
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  • For correspondence: denetclw@sfsu.edu
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Abstract

Heart development in the chicken embryo is regulated by a concert of cardiogenic morphogens and signaling molecules, but the physiological signal molecule nitric oxide(NO) has not been studied in the context of heart formation. A dynamic investigation of endoderm NO formation demonstrates for the first time a correlation with the established development events of the cardiac heart fields and heart tube. Manipulation of endoderm NO signaling demonstrate a role of NO signaling in the differentiation and proliferation of cardiac progenitors for heart tube formation and cardiac heart field development. To investigate NO in the proliferation of myocardial cells in the heart tube embryos, a computer vision based artificial intelligence approach is followed to automate the long and tedious job of counting cells in a large image dataset. We document NO as an important signaling molecule in the regulation of nascent embryonic cardiogenesis whose effects on other early cardiogenic morphogens is unknown.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://github.com/CCCofficial/cell-counting-chicken-embryo-myocardial

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted August 30, 2020.
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Endoderm Nitric Oxide Signals to Regulate Nascent Development of Cardiac Progenitors in Chicken Embryos
Devan H. Shah, Sujoy K. Biswas, Adrian M. Martin, Simone Bianco, Wilfred F. Denetclaw
bioRxiv 2020.08.29.272989; doi: https://doi.org/10.1101/2020.08.29.272989
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Endoderm Nitric Oxide Signals to Regulate Nascent Development of Cardiac Progenitors in Chicken Embryos
Devan H. Shah, Sujoy K. Biswas, Adrian M. Martin, Simone Bianco, Wilfred F. Denetclaw
bioRxiv 2020.08.29.272989; doi: https://doi.org/10.1101/2020.08.29.272989

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