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Sequence-encoded and Composition-dependent Protein-RNA Interactions Control Multiphasic Condensate Morphologies

View ORCID ProfileTaranpreet Kaur, Muralikrishna Raju, View ORCID ProfileIbraheem Alshareedah, Richoo B. Davis, View ORCID ProfileDavit A. Potoyan, View ORCID ProfilePriya R. Banerjee
doi: https://doi.org/10.1101/2020.08.30.273748
Taranpreet Kaur
aDepartment of Physics, University at Buffalo, Buffalo NY 14260, USA
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Muralikrishna Raju
bDepartment of Chemistry, Iowa State University, Ames IA 50011, USA
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Ibraheem Alshareedah
aDepartment of Physics, University at Buffalo, Buffalo NY 14260, USA
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Richoo B. Davis
aDepartment of Physics, University at Buffalo, Buffalo NY 14260, USA
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Davit A. Potoyan
bDepartment of Chemistry, Iowa State University, Ames IA 50011, USA
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  • For correspondence: prbanerj@buffalo.edu potoyan@iastate.edu
Priya R. Banerjee
aDepartment of Physics, University at Buffalo, Buffalo NY 14260, USA
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  • For correspondence: prbanerj@buffalo.edu potoyan@iastate.edu
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ABSTRACT

Multivalent protein-protein and protein-RNA interactions are the drivers of biological phase separation. Biomolecular condensates typically contain a dense network of multiple proteins and RNAs, and their competing molecular interactions play key roles in regulating the condensate composition and structure. Employing a ternary system comprising of a prion-like polypeptide (PLP), arginine-rich polypeptide (RRP), and RNA, we show that competition between the PLP and RNA for a single shared partner, the RRP, leads to RNA-induced demixing of PLP-RRP condensates into stable coexisting phases−homotypic PLP condensates and heterotypic RRP-RNA condensates. The morphology of these biphasic condensates (non-engulfing/ partial engulfing/ complete engulfing) is determined by the RNA-to-RRP stoichiometry and the hierarchy of intermolecular interactions, providing a glimpse of the broad range of multiphasic patterns that are accessible to these condensates. Our findings provide a minimal set of physical rules that govern the composition and spatial organization of multicomponent and multiphasic biomolecular condensates.

Competing Interest Statement

The authors have declared no competing interest.

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Posted December 10, 2020.
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Sequence-encoded and Composition-dependent Protein-RNA Interactions Control Multiphasic Condensate Morphologies
Taranpreet Kaur, Muralikrishna Raju, Ibraheem Alshareedah, Richoo B. Davis, Davit A. Potoyan, Priya R. Banerjee
bioRxiv 2020.08.30.273748; doi: https://doi.org/10.1101/2020.08.30.273748
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Sequence-encoded and Composition-dependent Protein-RNA Interactions Control Multiphasic Condensate Morphologies
Taranpreet Kaur, Muralikrishna Raju, Ibraheem Alshareedah, Richoo B. Davis, Davit A. Potoyan, Priya R. Banerjee
bioRxiv 2020.08.30.273748; doi: https://doi.org/10.1101/2020.08.30.273748

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