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Two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis thaliana

View ORCID ProfileRosa Pipitone, View ORCID ProfileSimona Eicke, View ORCID ProfileBarbara Pfister, View ORCID ProfileGaetan Glauser, View ORCID ProfileDenis Falconet, View ORCID ProfileClarisse Uwizeye, View ORCID ProfileThibaut Pralon, View ORCID ProfileSamuel Zeeman, View ORCID ProfileFelix Kessler, View ORCID ProfileEmilie Demarsy
doi: https://doi.org/10.1101/2020.08.30.274043
Rosa Pipitone
1Plant Physiology Laboratory, University of Neuchâtel, Neuchâtel, Switzerland
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Simona Eicke
2Institute of Molecular Plant Biology, Department of Biology ETH Zurich, Zurich, Switzerland
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Barbara Pfister
2Institute of Molecular Plant Biology, Department of Biology ETH Zurich, Zurich, Switzerland
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Gaetan Glauser
3Neuchâtel Platform of Analytical Chemistry, University of Neuchâtel, Neuchâtel, Switzerland
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Denis Falconet
4Univ. Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV, 38000 Grenoble, France
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Clarisse Uwizeye
4Univ. Grenoble Alpes, CNRS, CEA, INRAE, IRIG-DBSCI-LPCV, 38000 Grenoble, France
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Thibaut Pralon
1Plant Physiology Laboratory, University of Neuchâtel, Neuchâtel, Switzerland
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Samuel Zeeman
2Institute of Molecular Plant Biology, Department of Biology ETH Zurich, Zurich, Switzerland
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Felix Kessler
1Plant Physiology Laboratory, University of Neuchâtel, Neuchâtel, Switzerland
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  • For correspondence: emilie.demarsy@unige.ch felix.kessler@unine.ch
Emilie Demarsy
1Plant Physiology Laboratory, University of Neuchâtel, Neuchâtel, Switzerland
5Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva 4, Switzerland
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  • For correspondence: emilie.demarsy@unige.ch felix.kessler@unine.ch
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Abstract

Light triggers chloroplast differentiation whereby the etioplast transforms into a photosynthesizing chloroplast and the thylakoid rapidly emerges. However, the sequence of events during chloroplast differentiation remains poorly understood. Using Serial Block Face Scanning Electron Microscopy (SBF-SEM), we generated a series of chloroplast 3D reconstructions during differentiation, revealing chloroplast number and volume and the extent of envelope and thylakoid membrane surfaces. Furthermore, we used quantitative lipid and whole proteome data to complement the (ultra)structural data, providing a time-resolved, multi-dimensional description of chloroplast differentiation. This showed two distinct phases of chloroplast biogenesis: an initial photosynthesis-enabling ‘Structure Establishment Phase’ followed by a ‘Chloroplast Proliferation Phase’ during cell expansion. Moreover, these data detail thylakoid membrane expansion during de-etiolation at the seedling level and the relative contribution and differential regulation of proteins and lipids at each developmental stage. Altogether, we establish a roadmap for chloroplast differentiation, a critical process for plant photoautotrophic growth and survival.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Impact statement: Serial Block Face Scanning Electron Microscopy (SBF-SEM) associated with biomolecular analysis show that chloroplast differentiation proceeds by distinct ‘Structure Establishment’ and ‘Chloroplast Proliferation’ phases, each with differential protein and lipid regulation.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 01, 2020.
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Two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis thaliana
Rosa Pipitone, Simona Eicke, Barbara Pfister, Gaetan Glauser, Denis Falconet, Clarisse Uwizeye, Thibaut Pralon, Samuel Zeeman, Felix Kessler, Emilie Demarsy
bioRxiv 2020.08.30.274043; doi: https://doi.org/10.1101/2020.08.30.274043
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Two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis thaliana
Rosa Pipitone, Simona Eicke, Barbara Pfister, Gaetan Glauser, Denis Falconet, Clarisse Uwizeye, Thibaut Pralon, Samuel Zeeman, Felix Kessler, Emilie Demarsy
bioRxiv 2020.08.30.274043; doi: https://doi.org/10.1101/2020.08.30.274043

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