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Atomic structures of respiratory complex III2, complex IV and supercomplex III2-IV from vascular plants

View ORCID ProfileMaría Maldonado, Fei Guo, View ORCID ProfileJames A. Letts
doi: https://doi.org/10.1101/2020.08.30.274431
María Maldonado
1Department of Molecular and Cellular Biology, University of California Davis, 1 Shields Ave., Davis, CA, 95616, USA
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Fei Guo
1Department of Molecular and Cellular Biology, University of California Davis, 1 Shields Ave., Davis, CA, 95616, USA
2BIOEM Facility, University of California Davis, 1 Shields Ave., Davis, CA, 95616, USA
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James A. Letts
1Department of Molecular and Cellular Biology, University of California Davis, 1 Shields Ave., Davis, CA, 95616, USA
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  • For correspondence: jaletts@ucdavis.edu
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Abstract

Mitochondrial complex III (CIII2) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III2+IV), play key roles in respiration. However, structures of these plant complexes remain unknown. We present atomic models of CIII2, CIV and SC III2+IV from Vigna radiata determined by single-particle cryoEM. The structures reveal plant-specific differences in the MPP domain of CIII2 and define the subunit composition of CIV. Conformational heterogeneity analysis of CIII2 revealed long-range, coordinated movements across the complex, as well as the motion of CIII2’s iron-sulfur head domain. The CIV structure suggests that, in plants, proton translocation does not occur via the H-channel. The supercomplex interface differs significantly from that in yeast and bacteria in its interacting subunits, angle of approach and limited interactions in the mitochondrial matrix. These structures challenge long-standing assumptions about the plant complexes, generate new mechanistic hypotheses and allow for the generation of more selective agricultural inhibitors.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted August 31, 2020.
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Atomic structures of respiratory complex III2, complex IV and supercomplex III2-IV from vascular plants
María Maldonado, Fei Guo, James A. Letts
bioRxiv 2020.08.30.274431; doi: https://doi.org/10.1101/2020.08.30.274431
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Atomic structures of respiratory complex III2, complex IV and supercomplex III2-IV from vascular plants
María Maldonado, Fei Guo, James A. Letts
bioRxiv 2020.08.30.274431; doi: https://doi.org/10.1101/2020.08.30.274431

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