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Comparative analysis of human brain organoids of brainstem and midbrain at single-cell resolution

Kaoru Kinugawa, Joachim Luginbühl, Takeshi K. Matsui, Nobuyuki Eura, Yoshihiko M. Sakaguchi, Jay W Shin, Kazuma Sugie, Jens C. Schwamborn, View ORCID ProfileEiichiro Mori
doi: https://doi.org/10.1101/2020.09.02.279380
Kaoru Kinugawa
1Department of Neurology, Nara Medical University, Kashihara, Nara 634-8521, Japan
2Department of Future Basic Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan
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Joachim Luginbühl
3Laboratory for Advanced Genomics Circuit, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan
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Takeshi K. Matsui
1Department of Neurology, Nara Medical University, Kashihara, Nara 634-8521, Japan
2Department of Future Basic Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan
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Nobuyuki Eura
1Department of Neurology, Nara Medical University, Kashihara, Nara 634-8521, Japan
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Yoshihiko M. Sakaguchi
2Department of Future Basic Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan
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Jay W Shin
3Laboratory for Advanced Genomics Circuit, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan
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Kazuma Sugie
1Department of Neurology, Nara Medical University, Kashihara, Nara 634-8521, Japan
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Jens C. Schwamborn
4Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Belvaux L-4367, Luxembourg
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Eiichiro Mori
2Department of Future Basic Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan
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  • ORCID record for Eiichiro Mori
  • For correspondence: emori@naramed-u.ac.jp
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ABSTRACT

Human brain organoids provide us the means to investigate human brain development and neurological diseases, and single-cell RNA-sequencing (scRNA-seq) technologies allow us to identify homologous cell types and the molecular heterogeneity between individual cells. Previously established human brain organoids of brainstem (hBSOs) and midbrain (hMBOs) were analyzed by scRNA-seq, but the difference in cellular composition between these organoids remains unclear. Here, we integrated and compared the single-cell transcriptome of hBSOs and hMBOs. Our analysis demonstrated that the hBSOs and hMBOs contain some unique cell types, including inflammatory and mesenchymal cells. Further comparison of the hBSOs and hMBOs with publicly available scRNA-seq dataset of human fetal midbrain (hMB) showed high similarity in their neuronal components. These results provide new insights into human brain organoid technologies.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 03, 2020.
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Comparative analysis of human brain organoids of brainstem and midbrain at single-cell resolution
Kaoru Kinugawa, Joachim Luginbühl, Takeshi K. Matsui, Nobuyuki Eura, Yoshihiko M. Sakaguchi, Jay W Shin, Kazuma Sugie, Jens C. Schwamborn, Eiichiro Mori
bioRxiv 2020.09.02.279380; doi: https://doi.org/10.1101/2020.09.02.279380
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Comparative analysis of human brain organoids of brainstem and midbrain at single-cell resolution
Kaoru Kinugawa, Joachim Luginbühl, Takeshi K. Matsui, Nobuyuki Eura, Yoshihiko M. Sakaguchi, Jay W Shin, Kazuma Sugie, Jens C. Schwamborn, Eiichiro Mori
bioRxiv 2020.09.02.279380; doi: https://doi.org/10.1101/2020.09.02.279380

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