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Alcohol dependence promotes systemic IFN-γ and IL-17 responses in mice

View ORCID ProfileKayla Frank, Shawn Abeynaike, Rana Nikzad, Reesha R. Patel, Amanda J. Roberts, Marisa Roberto, View ORCID ProfileSilke Paust
doi: https://doi.org/10.1101/2020.09.03.280735
Kayla Frank
1Department of Immunology and Microbiology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA
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Shawn Abeynaike
1Department of Immunology and Microbiology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA
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Rana Nikzad
1Department of Immunology and Microbiology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA
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Reesha R. Patel
2Department of Molecular Medicine, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA
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Amanda J. Roberts
3Animal Models Core, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA
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Marisa Roberto
2Department of Molecular Medicine, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA
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Silke Paust
1Department of Immunology and Microbiology, The Scripps Research Institute, 10550 N. Torrey Pines Rd, La Jolla, CA 92037, USA
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  • ORCID record for Silke Paust
  • For correspondence: paust@scripps.edu
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Abstract

Alcohol use disorder (AUD) is a chronic relapsing disorder characterized by an impaired ability to stop or control alcohol use despite adverse social, occupational, or health consequences. AUD is associated with a variety of physiological changes and is a substantial risk factor for numerous diseases. We aimed to characterize systemic alterations in immune responses using a mouse model of chronic intermittent alcohol exposure to induce alcohol dependence. We exposed mice to chronic intermittent ethanol vapor for 4 weeks and used multiparametric flow cytometry to analyze the expression of cytokines IFN-γ, IL-4, IL-10, IL-12 and IL-17 by different immune cells in the blood, spleen and liver of alcohol dependent and non-dependent control mice. We found increases in IFN-γ and IL-17 expression in a cell type- and organ-specific manner. Often, B cells and neutrophils are primary contributors to increased IFN-γ and IL-17 levels while other cell types play a secondary role. We conclude that chronic alcohol exposure promotes systemic pro-inflammatory IFN-γ and IL-17 responses in mice. These responses are likely important in the development of alcohol-related diseases, but further characterization is necessary to understand the initiation and effects of systemic inflammatory responses to chronic alcohol exposure.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted September 03, 2020.
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Alcohol dependence promotes systemic IFN-γ and IL-17 responses in mice
Kayla Frank, Shawn Abeynaike, Rana Nikzad, Reesha R. Patel, Amanda J. Roberts, Marisa Roberto, Silke Paust
bioRxiv 2020.09.03.280735; doi: https://doi.org/10.1101/2020.09.03.280735
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Alcohol dependence promotes systemic IFN-γ and IL-17 responses in mice
Kayla Frank, Shawn Abeynaike, Rana Nikzad, Reesha R. Patel, Amanda J. Roberts, Marisa Roberto, Silke Paust
bioRxiv 2020.09.03.280735; doi: https://doi.org/10.1101/2020.09.03.280735

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