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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiation

View ORCID ProfileSusanna Fraguas, Sheila Cárcel, Coral Vivancos, View ORCID ProfileMa Dolores Molina, Jordi Ginés, Judith Mazariegos, Thileepan Sekaran, Kerstin Bartscherer, Rafael Romero, View ORCID ProfileFrancesc Cebrià
doi: https://doi.org/10.1101/2020.09.08.287045
Susanna Fraguas
1Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona
2Institute of Biomedicine of the University of Barcelona (IBUB)
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Sheila Cárcel
1Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona
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Coral Vivancos
1Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona
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Ma Dolores Molina
1Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona
2Institute of Biomedicine of the University of Barcelona (IBUB)
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Jordi Ginés
1Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona
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Judith Mazariegos
1Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona
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Thileepan Sekaran
4EMBL Heidelberg
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Kerstin Bartscherer
3Hubrecht Institute, The Netherlands
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Rafael Romero
1Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona
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Francesc Cebrià
1Department of Genetics, Microbiology and Statistics. Faculty of Biology, University of Barcelona
2Institute of Biomedicine of the University of Barcelona (IBUB)
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  • ORCID record for Francesc Cebrià
  • For correspondence: fcebrias@ub.edu
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ABSTRACT

The regulation of stem cells plasticity and differentiation is still an open question in developmental biology. CBP (CREB-binding protein)/p300 is a conserved gene family which functions as a transcriptional co-activator and shows an important role in a wide range of cellular processes, such as cell death, DNA damage response and tumorigenesis. Moreover, CBPs have an acetyl transferase activity that is relevant as histone and non-histone acetylation results in changes in chromatin architecture and protein activity that affects gene expression. Many studies have shown the conserved functions of CBP/p300 on stem cell proliferation and differentiation. The planarian Schmidtea mediterranea is an excellent model to study in vivo the molecular mechanism underlying stem cell differentiation during regeneration. We have identified five different Smed-cbp genes in S. mediterranea that show different expression patterns. Functional analyses indicate that Smed-cbp-2 seems to be essential for stem cell maintenance and cell survival. On the other hand, the silencing of Smed-cbp-3 results in the growth of apparently normal blastemas; however, these remain largely depigmented and undifferentiated. Smed-cbp-3 silencing affects the differentiation of several cell lineages including neural, epidermal, digestive and excretory cell types. Finally, we have analyzed the predicted interactomes of CBP-2 and CBP-3 as an initial step to better understand their function on planarian stem cell biology.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted September 08, 2020.
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiation
Susanna Fraguas, Sheila Cárcel, Coral Vivancos, Ma Dolores Molina, Jordi Ginés, Judith Mazariegos, Thileepan Sekaran, Kerstin Bartscherer, Rafael Romero, Francesc Cebrià
bioRxiv 2020.09.08.287045; doi: https://doi.org/10.1101/2020.09.08.287045
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiation
Susanna Fraguas, Sheila Cárcel, Coral Vivancos, Ma Dolores Molina, Jordi Ginés, Judith Mazariegos, Thileepan Sekaran, Kerstin Bartscherer, Rafael Romero, Francesc Cebrià
bioRxiv 2020.09.08.287045; doi: https://doi.org/10.1101/2020.09.08.287045

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