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Single-Cell Resolution of Lineage Trajectories in the Arabidopsis Stomatal Lineage and Developing Leaf

View ORCID ProfileCamila B. Lopez-Anido, View ORCID ProfileAnne Vatén, View ORCID ProfileNicole K. Smoot, View ORCID ProfileNidhi Sharma, View ORCID ProfileVictoria Guo, View ORCID ProfileYan Gong, View ORCID ProfileM. Ximena Anleu Gil, View ORCID ProfileAnnika K. Weimer, View ORCID ProfileDominique C. Bergmann
doi: https://doi.org/10.1101/2020.09.08.288498
Camila B. Lopez-Anido
1Department of Biology, Stanford University, Stanford, CA 94305-5020, USA
2Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305-5020, USA
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  • ORCID record for Camila B. Lopez-Anido
Anne Vatén
1Department of Biology, Stanford University, Stanford, CA 94305-5020, USA
3Organismal and Evolutionary Biology Research Programme, Biological and Environmental Sciences, University of Helsinki, Helsinki 00014, Finland
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Nicole K. Smoot
2Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305-5020, USA
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  • ORCID record for Nicole K. Smoot
Nidhi Sharma
2Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305-5020, USA
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Victoria Guo
2Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305-5020, USA
4Genentech Inc, 1 DNA Way, South San Francisco, CA 94080
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Yan Gong
1Department of Biology, Stanford University, Stanford, CA 94305-5020, USA
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M. Ximena Anleu Gil
2Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305-5020, USA
5Department of Plant Biology, 2203 Life Sciences Building, University of California, Davis, CA 95616, USA
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Annika K. Weimer
1Department of Biology, Stanford University, Stanford, CA 94305-5020, USA
6Department of Genetics, Stanford University School of Medicine, Stanford, California 94305, USA
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Dominique C. Bergmann
1Department of Biology, Stanford University, Stanford, CA 94305-5020, USA
2Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305-5020, USA
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  • For correspondence: bergmann@stanford.edu
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SUMMARY

Dynamic cell states underlie flexible developmental programs, such as with the stomatal lineage of the Arabidopsis epidermis. Initial stages of the lineage feature asynchronous and indeterminate divisions modulated by environmental cues, enabling cell fate flexibility to generate the requisite density and pattern of stomata for a given environment. It remains unclear, however, how flexibility of cell fates is controlled. Here, we uncovered distinct models of cell state differentiation within Arabidopsis leaf tissue by leveraging single-cell transcriptomics and molecular genetics. Our findings resolved underlying heterogeneity within cell states of the flexible epidermal stomatal lineage, which appear to exist along a continuum, with progressive cell specification. Beyond the early stages of the lineage, we discovered that the core transcriptional regulator SPEECHLESS is required for cell fate commitment to yield stomatal guard cells. Overall, our work has refined the stomatal lineage paradigm and uncovered progressive cell state decisions along lineage trajectories in developing leaves.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 09, 2020.
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Single-Cell Resolution of Lineage Trajectories in the Arabidopsis Stomatal Lineage and Developing Leaf
Camila B. Lopez-Anido, Anne Vatén, Nicole K. Smoot, Nidhi Sharma, Victoria Guo, Yan Gong, M. Ximena Anleu Gil, Annika K. Weimer, Dominique C. Bergmann
bioRxiv 2020.09.08.288498; doi: https://doi.org/10.1101/2020.09.08.288498
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Single-Cell Resolution of Lineage Trajectories in the Arabidopsis Stomatal Lineage and Developing Leaf
Camila B. Lopez-Anido, Anne Vatén, Nicole K. Smoot, Nidhi Sharma, Victoria Guo, Yan Gong, M. Ximena Anleu Gil, Annika K. Weimer, Dominique C. Bergmann
bioRxiv 2020.09.08.288498; doi: https://doi.org/10.1101/2020.09.08.288498

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