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Future-proofing code: Principles of coding for plant ecophysiology with {photosynthesis} as a case study

View ORCID ProfileJoseph R. Stinziano, Cassaundra Roback, View ORCID ProfileDemi Gamble, View ORCID ProfileBridget K. Murphy, View ORCID ProfilePatrick J. Hudson, View ORCID ProfileChristopher D. Muir
doi: https://doi.org/10.1101/2020.09.11.293530
Joseph R. Stinziano
1Department of Biology, University of New Mexico, Albuquerque, NM, USA 87131-0001
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  • For correspondence: josephstinziano@gmail.com
Cassaundra Roback
1Department of Biology, University of New Mexico, Albuquerque, NM, USA 87131-0001
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Demi Gamble
2CSIRO Agriculture and Food, Myall Vale, NSW, Australia
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Bridget K. Murphy
3Department of Biology, University of Toronto Mississauga, Mississauga, ON, Canada
4Graduate Program in Cell and Systems Biology, University of Toronto, Toronto, ON, Canada
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Patrick J. Hudson
1Department of Biology, University of New Mexico, Albuquerque, NM, USA 87131-0001
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Christopher D. Muir
5School of Life Sciences, University of Hawai’i at Manoa, Honolulu, HI, USA
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Summary

  • Plant physiological ecology is founded on a rich body of physical and chemical theory, but it is challenging to connect theory with data in unambiguous, analytically rigorous, and reproducible ways. Custom scripts written in computer programming languages (coding) enable plant ecophysiologists to model plant processes and fit models to data reproducibly using advanced statistical techniques. Since most ecophysiologists lack formal programming education, we have yet to adopt a unified set of coding principles and standards that could make coding easier to learn, use, and modify.

  • We outline principles and standards for coding in plant ecophysiology to develop: 1) standardized nomenclature, 2) consistency in style, 3) increased modularity/extensibility for easier editing and understanding; 4) code scalability for application to large datasets, 5) documented contingencies for code maintenance; 6) documentation to facilitate user understanding; and 7) extensive tutorials for biologists new to coding to rapidly become proficient with software.

  • We illustrate these principles using a new R package, {photosynthesis}, designed to provide a set of analytical tools for plant ecophysiology.

  • Our goal with these principles is to future-proof coding efforts to ensure new advances and analytical tools can be rapidly incorporated into the field, while ensuring software maintenance across scientific generations.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://github.com/cdmuir/photosynthesis

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted September 12, 2020.
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Future-proofing code: Principles of coding for plant ecophysiology with {photosynthesis} as a case study
Joseph R. Stinziano, Cassaundra Roback, Demi Gamble, Bridget K. Murphy, Patrick J. Hudson, Christopher D. Muir
bioRxiv 2020.09.11.293530; doi: https://doi.org/10.1101/2020.09.11.293530
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Future-proofing code: Principles of coding for plant ecophysiology with {photosynthesis} as a case study
Joseph R. Stinziano, Cassaundra Roback, Demi Gamble, Bridget K. Murphy, Patrick J. Hudson, Christopher D. Muir
bioRxiv 2020.09.11.293530; doi: https://doi.org/10.1101/2020.09.11.293530

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