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Characterization of Wnt Signaling Genes in Diaphorina citri, Asian Citrus Psyllid

View ORCID ProfileChad Vosburg, Max Reynolds, Rita Noel, View ORCID ProfileTeresa Shippy, View ORCID ProfilePrashant S Hosmani, View ORCID ProfileMirella Flores-Gonzalez, View ORCID ProfileLukas A Mueller, View ORCID ProfileWayne B Hunter, View ORCID ProfileSusan J Brown, View ORCID ProfileTom D’Elia, View ORCID ProfileSurya Saha
doi: https://doi.org/10.1101/2020.09.21.306100
Chad Vosburg
1Indian River State College, Fort Pierce, FL 34981
2Department of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA 16802
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Max Reynolds
1Indian River State College, Fort Pierce, FL 34981
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Rita Noel
1Indian River State College, Fort Pierce, FL 34981
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Teresa Shippy
3KSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS
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Prashant S Hosmani
4Boyce Thompson Institute, Ithaca, NY 14853
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Mirella Flores-Gonzalez
4Boyce Thompson Institute, Ithaca, NY 14853
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Lukas A Mueller
4Boyce Thompson Institute, Ithaca, NY 14853
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Wayne B Hunter
5USDA-ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945
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Susan J Brown
3KSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS
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Tom D’Elia
1Indian River State College, Fort Pierce, FL 34981
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Surya Saha
4Boyce Thompson Institute, Ithaca, NY 14853
6Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721
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  • For correspondence: ss2489@cornell.edu
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Abstract

The Asian citrus psyllid, Diaphorina citri, is an insect vector that transmits Candidatus Liberibacter asiaticus, the causal agent of the Huanglongbing (HLB) or citrus greening disease. This disease has devastated Florida’s citrus industry and threatens California’s industry as well as other citrus producing regions around the world. To find novel solutions to the disease, a better understanding of the vector is needed. The D. citri genome has been used to identify and characterize genes involved in Wnt signaling pathways. Wnt signaling is utilized for many important biological processes in metazoans, such as patterning and tissue generation. Curation based on RNA sequencing data and sequence homology confirm twenty four Wnt signaling genes within the D. citri genome, including homologs for beta-catenin, Frizzled receptors, and seven Wnt-ligands. Through phylogenetic analysis, we classify D. citri Wnt-ligands as Wg/Wnt1, Wnt5, Wnt6, Wnt7, Wnt10, Wnt11, and WntA. The D. citri version 3.0 genome with chromosomal length scaffolds reveals a conserved Wnt1-Wnt6-Wnt10 gene cluster with gene configuration similar to that in Drosophila melanogaster. These findings provide a greater insight into the evolutionary history of D. citri and Wnt signaling in this important hemipteran vector. Manual annotation was essential for identifying high quality gene models. These gene models can further be used to develop molecular systems, such as CRISPR and RNAi, that target and control D. citri populations, to manage the spread of HLB. Manual annotation of Wnt signaling pathways was done as part of a collaborative community annotation project (https://citrusgreening.org/annotation/index).

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • The abstract, references and methods have been updated.

  • https://citrusgreening.org/annotation/index

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted December 13, 2020.
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Characterization of Wnt Signaling Genes in Diaphorina citri, Asian Citrus Psyllid
Chad Vosburg, Max Reynolds, Rita Noel, Teresa Shippy, Prashant S Hosmani, Mirella Flores-Gonzalez, Lukas A Mueller, Wayne B Hunter, Susan J Brown, Tom D’Elia, Surya Saha
bioRxiv 2020.09.21.306100; doi: https://doi.org/10.1101/2020.09.21.306100
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Characterization of Wnt Signaling Genes in Diaphorina citri, Asian Citrus Psyllid
Chad Vosburg, Max Reynolds, Rita Noel, Teresa Shippy, Prashant S Hosmani, Mirella Flores-Gonzalez, Lukas A Mueller, Wayne B Hunter, Susan J Brown, Tom D’Elia, Surya Saha
bioRxiv 2020.09.21.306100; doi: https://doi.org/10.1101/2020.09.21.306100

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