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Humoral response to SARS-CoV-2 by healthy and sick dogs during COVID-19 pandemic in Spain

View ORCID ProfileAna Judith Perisé-Barrios, View ORCID ProfileBeatriz Davinia Tomeo-Martín, Pablo Gómez-Ochoa, Pablo Delgado-Bonet, Pedro Plaza, Paula Palau-Concejo, Jorge González, Gustavo Ortiz-Diez, Antonio Meléndez-Lazo, Michaela Gentil, Javier García-Castro, Alicia Barbero-Fernández
doi: https://doi.org/10.1101/2020.09.22.308023
Ana Judith Perisé-Barrios
1Biomedical Research Unit, Universidad Alfonso X el Sabio, Avda Universidad 1, 28691 Villanueva de la Cañada (Madrid), Spain
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  • ORCID record for Ana Judith Perisé-Barrios
  • For correspondence: aperibar@uax.es aabarbero@uax.es
Beatriz Davinia Tomeo-Martín
1Biomedical Research Unit, Universidad Alfonso X el Sabio, Avda Universidad 1, 28691 Villanueva de la Cañada (Madrid), Spain
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Pablo Gómez-Ochoa
2Vetcorner, Calle Mosén José Bosqued 2, 50012 Zaragoza, Spain
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Pablo Delgado-Bonet
1Biomedical Research Unit, Universidad Alfonso X el Sabio, Avda Universidad 1, 28691 Villanueva de la Cañada (Madrid), Spain
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Pedro Plaza
3ERVET-DIEZ BRU, Calle Tordesillas 4, 28925 Alcorcón (Madrid), Spain
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Paula Palau-Concejo
1Biomedical Research Unit, Universidad Alfonso X el Sabio, Avda Universidad 1, 28691 Villanueva de la Cañada (Madrid), Spain
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Jorge González
4Micros Veterinaria SL, Calle Profesor Pedro Cármenes, 24007 León, Spain
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Gustavo Ortiz-Diez
5Faculty of Veterinary, Universidad Alfonso X el Sabio, Avda Universidad 1, 28691 Villanueva de la Cañada (Madrid), Spain
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Antonio Meléndez-Lazo
6Laboklin GmbH & Co. KG, Steubenstraße 4, 97688 Bad Kissingen, Germany
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Michaela Gentil
6Laboklin GmbH & Co. KG, Steubenstraße 4, 97688 Bad Kissingen, Germany
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Javier García-Castro
7Cellular Biotechnology Unit, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo, Km 2, 28220 Majadahonda (Madrid), Spain
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Alicia Barbero-Fernández
5Faculty of Veterinary, Universidad Alfonso X el Sabio, Avda Universidad 1, 28691 Villanueva de la Cañada (Madrid), Spain
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  • For correspondence: aperibar@uax.es aabarbero@uax.es
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Summary

COVID-19 is a zoonotic disease originated by SARS-CoV-2. Infection of animals with SARS-CoV-2 are being reported during last months, and also an increase of severe lung pathologies in domestic dogs has been detected by veterinarians in Spain. Therefore it is necessary to describe the pathological processes in those animals that show symptoms similar to those described in humans affected by COVID-19. The potential for companion animals contributing to the continued human-to-human disease, infectivity, and community spread is an urgent issue to be considered.

Forty animals with pulmonary pathologies were studied by chest X-ray, ultrasound study, and computed tomography. Nasopharyngeal and rectal swab were analyzed to detect canine pathogens, including SARS-CoV-2. Twenty healthy dogs living in SARS-CoV-2 positive households were included. Immunoglobulin detection by different immunoassays was performed. Our findings show that sick dogs presented severe alveolar or interstitial pattern, with pulmonary opacity, parenchymal abnormalities, and bilateral lesions. Forty dogs were negative for SARS-CoV-2 but Mycoplasma spp. was detected in 26 of 33 dogs. Five healthy and one pathological dog presented IgG against SARS-CoV-2.

Here we report that despite detecting dogs with IgG α-SARS-CoV-2, we never obtained a positive RT-qPCR, not even in dogs with severe pulmonary disease; suggesting that even in the case of a canine infection transmission would be unlikely. Moreover, dogs living in COVID-19 positive households could have been more exposed to be infected during outbreaks.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Funding Study funded by Fundación Universidad Alfonso X el Sabio (1.011.115 grant to A.J.P-B.; 1.011.101 to A.B-F.) and Comunidad de Madrid (COV20/01398 grant to A.J.P-B. and A.B-F.).

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted September 22, 2020.
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Humoral response to SARS-CoV-2 by healthy and sick dogs during COVID-19 pandemic in Spain
Ana Judith Perisé-Barrios, Beatriz Davinia Tomeo-Martín, Pablo Gómez-Ochoa, Pablo Delgado-Bonet, Pedro Plaza, Paula Palau-Concejo, Jorge González, Gustavo Ortiz-Diez, Antonio Meléndez-Lazo, Michaela Gentil, Javier García-Castro, Alicia Barbero-Fernández
bioRxiv 2020.09.22.308023; doi: https://doi.org/10.1101/2020.09.22.308023
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Humoral response to SARS-CoV-2 by healthy and sick dogs during COVID-19 pandemic in Spain
Ana Judith Perisé-Barrios, Beatriz Davinia Tomeo-Martín, Pablo Gómez-Ochoa, Pablo Delgado-Bonet, Pedro Plaza, Paula Palau-Concejo, Jorge González, Gustavo Ortiz-Diez, Antonio Meléndez-Lazo, Michaela Gentil, Javier García-Castro, Alicia Barbero-Fernández
bioRxiv 2020.09.22.308023; doi: https://doi.org/10.1101/2020.09.22.308023

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