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Transsynaptic mapping of Drosophila mushroom body output neurons

View ORCID ProfileKristin M Scaplen, View ORCID ProfileMustafa Talay, John D Fisher, Raphael Cohn, Altar Sorkaç, View ORCID ProfileYoshinori Aso, View ORCID ProfileGilad Barnea, View ORCID ProfileKarla R Kaun
doi: https://doi.org/10.1101/2020.09.22.309021
Kristin M Scaplen
1Department of Neuroscience, Brown University, Providence, RI 02912 USA
2Department of Psychology, Bryant University, Smithfield RI 02917, USA
5Center for Health and Behavioral Sciences, Bryant University, Smithfield, RI 02917 USA
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  • For correspondence: kscaplen@bryant.edu gilad_barnea@brown.edu karla_kaun@brown.edu
Mustafa Talay
1Department of Neuroscience, Brown University, Providence, RI 02912 USA
6Howard Hughes Medical Institute, Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, 02138, USA
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John D Fisher
1Department of Neuroscience, Brown University, Providence, RI 02912 USA
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Raphael Cohn
3Laboratory of Neurophysiology and Behavior, The Rockefeller University, New York, NY 10065, USA
7Department of Biological Studies, Columbia University, New York, NY, 10027
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Altar Sorkaç
1Department of Neuroscience, Brown University, Providence, RI 02912 USA
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Yoshinori Aso
4Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States
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Gilad Barnea
1Department of Neuroscience, Brown University, Providence, RI 02912 USA
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  • For correspondence: kscaplen@bryant.edu gilad_barnea@brown.edu karla_kaun@brown.edu
Karla R Kaun
1Department of Neuroscience, Brown University, Providence, RI 02912 USA
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  • For correspondence: kscaplen@bryant.edu gilad_barnea@brown.edu karla_kaun@brown.edu
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Abstract

The Mushroom Body (MB) is a well-characterized associative memory structure within the Drosophila brain. Although previous studies have analyzed MB connectivity and provided a map of inputs and outputs, a detailed map of the downstream targets is missing. Using the genetic anterograde transsynaptic tracing tool, trans-Tango, we identified divergent projections across the brain and convergent downstream targets of the MB output neurons (MBONs). Our analysis revealed at least three separate targets that receive convergent input from MBONs: other MBONs, the fan shaped body (FSB), and the lateral accessory lobe (LAL). We describe, both anatomically and functionally, a multilayer circuit in which inhibitory and excitatory MBONs converge on the same genetic subset of FSB and LAL neurons. This circuit architecture provides an opportunity for the brain to update information and integrate it with previous experience before executing appropriate behavioral responses.

Highlights -The postsynaptic connections of the output neurons of the mushroom body, a structure that integrates environmental cues with associated valence, are mapped using trans-Tango.

-Mushroom body circuits are highly interconnected with several points of convergence among mushroom body output neurons (MBONs).

-The postsynaptic partners of MBONs have divergent projections across the brain and convergent projections to select target neuropils outside the mushroom body important for multimodal integration.

-Functional connectivity suggests the presence of multisynaptic pathways that have several layers of integration prior to initiation of an output response.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 23, 2020.
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Transsynaptic mapping of Drosophila mushroom body output neurons
Kristin M Scaplen, Mustafa Talay, John D Fisher, Raphael Cohn, Altar Sorkaç, Yoshinori Aso, Gilad Barnea, Karla R Kaun
bioRxiv 2020.09.22.309021; doi: https://doi.org/10.1101/2020.09.22.309021
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Transsynaptic mapping of Drosophila mushroom body output neurons
Kristin M Scaplen, Mustafa Talay, John D Fisher, Raphael Cohn, Altar Sorkaç, Yoshinori Aso, Gilad Barnea, Karla R Kaun
bioRxiv 2020.09.22.309021; doi: https://doi.org/10.1101/2020.09.22.309021

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