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Chitin Biosynthesis Genes in Diaphorina citri

Sherry Miller, View ORCID ProfileBlessy Tamayo, View ORCID ProfileTeresa D. Shippy, View ORCID ProfilePrashant S Hosmani, View ORCID ProfileMirella Flores-Gonzalez, View ORCID ProfileLukas A Mueller, View ORCID ProfileWayne B Hunter, View ORCID ProfileSusan J Brown, View ORCID ProfileTom D’elia, View ORCID ProfileSurya Saha
doi: https://doi.org/10.1101/2020.09.22.309211
Sherry Miller
1Division of Biology, Kansas State University, Manhattan, KS 66506
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Blessy Tamayo
2Indian River State College, Fort Pierce, FL 34981
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Teresa D. Shippy
1Division of Biology, Kansas State University, Manhattan, KS 66506
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Prashant S Hosmani
3Boyce Thompson Institute, Ithaca, NY 14853
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Mirella Flores-Gonzalez
3Boyce Thompson Institute, Ithaca, NY 14853
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Lukas A Mueller
3Boyce Thompson Institute, Ithaca, NY 14853
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Wayne B Hunter
4USDA-ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945
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Susan J Brown
1Division of Biology, Kansas State University, Manhattan, KS 66506
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Tom D’elia
2Indian River State College, Fort Pierce, FL 34981
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Surya Saha
3Boyce Thompson Institute, Ithaca, NY 14853
5Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721
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  • For correspondence: ss2489@cornell.edu
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Abstract

The polysaccharide chitin is critical for the formation of many insect structures, including the exoskeleton, and is required for normal development. Here we report the annotation of three genes from the chitin synthesis pathway in the Asian citrus psyllid, Diaphorina citri. Most insects have two chitin synthase (CHS) genes but, like other hemipterans, D. citri has only one. In contrast, D. citri is unusual among insects in having two UDP-N-acetylglucosamine pyrophosphorylase (UAP) genes. One of the D. citri UAP genes is broadly expressed, while the other is expressed predominantly in males. Our work helps pave the way for potential utilization of these genes as pest control targets.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Additional details have been added about the methods used.

  • https://citrusgreening.org/annotation/index

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted September 26, 2020.
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Chitin Biosynthesis Genes in Diaphorina citri
Sherry Miller, Blessy Tamayo, Teresa D. Shippy, Prashant S Hosmani, Mirella Flores-Gonzalez, Lukas A Mueller, Wayne B Hunter, Susan J Brown, Tom D’elia, Surya Saha
bioRxiv 2020.09.22.309211; doi: https://doi.org/10.1101/2020.09.22.309211
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Chitin Biosynthesis Genes in Diaphorina citri
Sherry Miller, Blessy Tamayo, Teresa D. Shippy, Prashant S Hosmani, Mirella Flores-Gonzalez, Lukas A Mueller, Wayne B Hunter, Susan J Brown, Tom D’elia, Surya Saha
bioRxiv 2020.09.22.309211; doi: https://doi.org/10.1101/2020.09.22.309211

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