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Reduction of visual stimulus artifacts using a spherical tank for small, aquatic animals

Kun Wang, Burkhard Arrenberg, Julian Hinz, View ORCID ProfileAristides B Arrenberg
doi: https://doi.org/10.1101/2020.09.22.309419
Kun Wang
1Werner Reichardt Centre for Integrative Neuroscience, Institute for Neurobiology, University of Tübingen, D-72076 Tübingen, Germany
2Graduate Training Centre for Neuroscience, University of Tübingen, D-72076 Tübingen, Germany
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Burkhard Arrenberg
3Prudenter Agas Hamburg, 22149 Hamburg, Germany
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Julian Hinz
1Werner Reichardt Centre for Integrative Neuroscience, Institute for Neurobiology, University of Tübingen, D-72076 Tübingen, Germany
2Graduate Training Centre for Neuroscience, University of Tübingen, D-72076 Tübingen, Germany
4Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
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Aristides B Arrenberg
1Werner Reichardt Centre for Integrative Neuroscience, Institute for Neurobiology, University of Tübingen, D-72076 Tübingen, Germany
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  • ORCID record for Aristides B Arrenberg
  • For correspondence: aristides.arrenberg@uni-tuebingen.de
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Abstract

Delivering appropriate stimuli remains a challenge in vision research, particularly for aquatic animals such as zebrafish. Due to the shape of the water tank and the associated optical paths of light rays, the stimulus can be subject to unwanted refraction or reflection artifacts, which may spoil the experiment and result in wrong conclusions. Here, we employ computer graphics simulations and calcium imaging in the zebrafish optic tectum to show, how a spherical glass container optically outperforms many previously used water containers, including Petri dish lids. We demonstrate that aquatic vision experiments suffering from total internal reflection artifacts at the water surface or at the flat container bottom may result in the erroneous detection of visual neurons with bipartite receptive fields and in the apparent absence of neurons selective for vertical motion. Our results and demonstrations will help aquatic vision neuroscientists on optimizing their stimulation setups.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 23, 2020.
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Reduction of visual stimulus artifacts using a spherical tank for small, aquatic animals
Kun Wang, Burkhard Arrenberg, Julian Hinz, Aristides B Arrenberg
bioRxiv 2020.09.22.309419; doi: https://doi.org/10.1101/2020.09.22.309419
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Reduction of visual stimulus artifacts using a spherical tank for small, aquatic animals
Kun Wang, Burkhard Arrenberg, Julian Hinz, Aristides B Arrenberg
bioRxiv 2020.09.22.309419; doi: https://doi.org/10.1101/2020.09.22.309419

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