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Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization

View ORCID ProfileAnabel-Lise Le Roux, Caterina Tozzi, Nikhil Walani, Xarxa Quiroga, Dobryna Zalvidea, Xavier Trepat, Margarita Staykova, Marino Arroyo, Pere Roca-Cusachs
doi: https://doi.org/10.1101/2020.09.23.310169
Anabel-Lise Le Roux
1Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST), 08028 Barcelona, Spain
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  • ORCID record for Anabel-Lise Le Roux
  • For correspondence: proca@ibecbarcelona.eu
Caterina Tozzi
2Universitat Politècnica de Catalunya (UPC), Campus Nord, Carrer de Jordi Girona, 1, 3, 08034 Barcelona
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Nikhil Walani
2Universitat Politècnica de Catalunya (UPC), Campus Nord, Carrer de Jordi Girona, 1, 3, 08034 Barcelona
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Xarxa Quiroga
1Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST), 08028 Barcelona, Spain
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Dobryna Zalvidea
1Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST), 08028 Barcelona, Spain
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Xavier Trepat
1Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST), 08028 Barcelona, Spain
3Universitat de Barcelona, 08036 Barcelona, Spain
4Institució Catalana de Recerca i Estudis Avançats (ICREA), Passeig de Lluís Companys, 23, 08010 Barcelona
5Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 08028 Barcelona, Spain
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Margarita Staykova
6Department of Physics, University of Durham, UK
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Marino Arroyo
1Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST), 08028 Barcelona, Spain
2Universitat Politècnica de Catalunya (UPC), Campus Nord, Carrer de Jordi Girona, 1, 3, 08034 Barcelona
7Centre International de Mètodes Numèrics en Enginyeria (CIMNE), 08034 Barcelona, Spain
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  • For correspondence: proca@ibecbarcelona.eu
Pere Roca-Cusachs
1Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST), 08028 Barcelona, Spain
3Universitat de Barcelona, 08036 Barcelona, Spain
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  • For correspondence: proca@ibecbarcelona.eu
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Abstract

In many physiological situations, BAR proteins interact with, and reshape, pre-existing curved membranes, contributing to essential cellular processes. However, the non-equilibrium and timedependent process of reshaping, and its dependence on initial membrane shape, remains largely unknown. Here we explain, both experimentally and through modelling, how a BAR protein dynamically interacts with mechanically bent lipid membranes. We capture protein binding to curved membranes, and characterize a variety of dynamical reshaping events depending on membrane shape and protein arrangement. The events can be generally understood by an isotropic-to-nematic phase transition, in which low curvature templates with isotropic protein orientation progress towards highly curved lipid tubes with nematic protein arrangement. Our findings also apply in cells, where mechanical stretch triggers BAR-protein-membrane interactions that enable potential mechanotransduction mechanisms. Our results characterize and broaden the reshaping processes of BAR proteins on mechanically constrained membranes, demonstrating the interplay between membrane mechanical stimuli and BAR protein response.

Competing Interest Statement

The authors have declared no competing interest.

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Posted September 25, 2020.
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Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization
Anabel-Lise Le Roux, Caterina Tozzi, Nikhil Walani, Xarxa Quiroga, Dobryna Zalvidea, Xavier Trepat, Margarita Staykova, Marino Arroyo, Pere Roca-Cusachs
bioRxiv 2020.09.23.310169; doi: https://doi.org/10.1101/2020.09.23.310169
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Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization
Anabel-Lise Le Roux, Caterina Tozzi, Nikhil Walani, Xarxa Quiroga, Dobryna Zalvidea, Xavier Trepat, Margarita Staykova, Marino Arroyo, Pere Roca-Cusachs
bioRxiv 2020.09.23.310169; doi: https://doi.org/10.1101/2020.09.23.310169

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