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Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis

Tania Moreno-Mármol, Mario Ledesma-Terrón, Noemí Tabanera, María Jesús Martin-Bermejo, Marcos J Cardozo, Florencia Cavodeassi, Paola Bovolenta
doi: https://doi.org/10.1101/2020.09.23.310631
Tania Moreno-Mármol
1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, c/ Nicolás Cabrera, 1, Campus de la Universidad Autónoma de Madrid, Madrid 28049, Spain
2CIBER de Enfermedades Raras (CIBERER) Madrid 28049, Spain
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Mario Ledesma-Terrón
1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, c/ Nicolás Cabrera, 1, Campus de la Universidad Autónoma de Madrid, Madrid 28049, Spain
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Noemí Tabanera
1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, c/ Nicolás Cabrera, 1, Campus de la Universidad Autónoma de Madrid, Madrid 28049, Spain
2CIBER de Enfermedades Raras (CIBERER) Madrid 28049, Spain
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María Jesús Martin-Bermejo
1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, c/ Nicolás Cabrera, 1, Campus de la Universidad Autónoma de Madrid, Madrid 28049, Spain
2CIBER de Enfermedades Raras (CIBERER) Madrid 28049, Spain
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Marcos J Cardozo
1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, c/ Nicolás Cabrera, 1, Campus de la Universidad Autónoma de Madrid, Madrid 28049, Spain
2CIBER de Enfermedades Raras (CIBERER) Madrid 28049, Spain
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Florencia Cavodeassi
1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, c/ Nicolás Cabrera, 1, Campus de la Universidad Autónoma de Madrid, Madrid 28049, Spain
2CIBER de Enfermedades Raras (CIBERER) Madrid 28049, Spain
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Paola Bovolenta
1Centro de Biología Molecular Severo Ochoa, CSIC-UAM, c/ Nicolás Cabrera, 1, Campus de la Universidad Autónoma de Madrid, Madrid 28049, Spain
2CIBER de Enfermedades Raras (CIBERER) Madrid 28049, Spain
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  • For correspondence: pbovolenta@cbm.csic.es
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Abstract

The vertebrate eye primordium consists of a pseudostratified neuroepithelium, the optic vesicle (OV), in which cells acquire neural retina or retinal pigment epithelium (RPE) fates. As these fates arise, the OV assumes a cup-shape, influenced by mechanical forces generated within the neural retina. Whether the RPE passively adapts to retinal changes or actively contributes to OV morphogenesis remains unexplored. Here, we generated a zebrafish Tg(E1-bhlhe40:GFP) line to track RPE morphogenesis and interrogate its participation in OV folding. We show that, in virtual absence of proliferation, RPE cells stretch into a squamous configuration, thereby matching the curvature of the underlying retina. Forced proliferation and localized interference with the RPE cytoskeleton disrupt its stretching and OV folding. Thus, extreme RPE flattening and accelerated differentiation are efficient solutions adopted by fast-developing species to enable timely optic cup formation. This mechanism differs in amniotes, in which proliferation largely drives RPE expansion with a much-reduced need of cell flattening.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • mistake in authros order

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license.
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Posted September 25, 2020.
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Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
Tania Moreno-Mármol, Mario Ledesma-Terrón, Noemí Tabanera, María Jesús Martin-Bermejo, Marcos J Cardozo, Florencia Cavodeassi, Paola Bovolenta
bioRxiv 2020.09.23.310631; doi: https://doi.org/10.1101/2020.09.23.310631
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Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis
Tania Moreno-Mármol, Mario Ledesma-Terrón, Noemí Tabanera, María Jesús Martin-Bermejo, Marcos J Cardozo, Florencia Cavodeassi, Paola Bovolenta
bioRxiv 2020.09.23.310631; doi: https://doi.org/10.1101/2020.09.23.310631

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