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Super-relaxed state of myosin in human skeletal muscle is fiber-type dependent

Lien A. Phung, Aurora D. Foster, Mark S. Miller, Dawn A. Lowe, View ORCID ProfileDavid D. Thomas
doi: https://doi.org/10.1101/2020.09.24.311795
Lien A. Phung
1Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, 55455
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Aurora D. Foster
2Department of Kinesiology, University of Massachusetts, Amherst, MA, 01003
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Mark S. Miller
2Department of Kinesiology, University of Massachusetts, Amherst, MA, 01003
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Dawn A. Lowe
3Department of Rehabilitation Medicine, University of Minnesota, Minneapolis, MN, 55455
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  • For correspondence: ddt@umn.edu lowex017@umn.edu
David D. Thomas
1Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, 55455
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  • ORCID record for David D. Thomas
  • For correspondence: ddt@umn.edu lowex017@umn.edu
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Abstract

The myosin super-relaxed state (SRX) in skeletal muscle is hypothesized to play an important role in regulating muscle contractility and thermogenesis in humans, but has only been examined in model organisms. Here we report the first human skeletal muscle SRX measurements, using quantitative epifluorescence microscopy of fluorescent 2’/3’-O-(N-methylanthraniloyl) ATP (mantATP) single-nucleotide turnover. Myosin heavy chain (MHC) isoform expression was determined using gel electrophoresis for each permeabilized vastus lateralis fiber, to allow for novel comparisons of SRX between fiber-types. We find that the fraction of myosin in SRX is less in MHC IIA fibers than in MHC I and IIAX fibers (p = 0.008). ATP turnover of SRX is faster in MHC IIAX fibers compared to MHC I and IIA fibers (p = 0.001). We conclude that SRX biochemistry is measurable in human skeletal muscle, and our data indicate that SRX depends on fiber type as classified by MHC isoform. Extension from this preliminary work would provide further understanding regarding the role of SRX in human muscle physiology.

Competing Interest Statement

The authors have declared no competing interest.

  • List of Abbreviations

    DRX
    Disordered relaxed state
    mantATP
    2’-/3’-O-(N’-Methylanthraniloy)-adenosine-5’-O-triphosphate
    MHC
    Myosin heavy chain
    SRX
    Super-relaxed state
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    Posted September 25, 2020.
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    Super-relaxed state of myosin in human skeletal muscle is fiber-type dependent
    Lien A. Phung, Aurora D. Foster, Mark S. Miller, Dawn A. Lowe, David D. Thomas
    bioRxiv 2020.09.24.311795; doi: https://doi.org/10.1101/2020.09.24.311795
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    Super-relaxed state of myosin in human skeletal muscle is fiber-type dependent
    Lien A. Phung, Aurora D. Foster, Mark S. Miller, Dawn A. Lowe, David D. Thomas
    bioRxiv 2020.09.24.311795; doi: https://doi.org/10.1101/2020.09.24.311795

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