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Hamster and ferret experimental infection with intranasal low dose of a single strain of SARS-CoV-2

Elodie Monchatre-Leroy, Sandrine Lesellier, Marine Wasniewski, Evelyne Picard-Meyer, Céline Richomme, Franck Boué, Sandra Lacôte, Séverine Murri, Coralie Pulido, Johann Vulin, Francisco J Salguero, Meriadeg Ar Gouilh, Alexandre Servat, Philippe Marianneau
doi: https://doi.org/10.1101/2020.09.24.311977
Elodie Monchatre-Leroy
1Nancy laboratory for rabies and wildlife, ANSES, Malzéville, France
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  • For correspondence: elodie.monchatre-leroy@anses.fr
Sandrine Lesellier
2Nancy laboratory for rabies and wildlife, ANSES, Atton experimental facility, Atton France
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Marine Wasniewski
3Nancy laboratory for rabies and wildlife, ANSES, Lyssavirus Unit, Malzéville, France
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Evelyne Picard-Meyer
3Nancy laboratory for rabies and wildlife, ANSES, Lyssavirus Unit, Malzéville, France
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Céline Richomme
4Nancy laboratory for rabies and wildlife, ANSES, SEEpiAS Unit, Malzéville, France
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Franck Boué
4Nancy laboratory for rabies and wildlife, ANSES, SEEpiAS Unit, Malzéville, France
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Sandra Lacôte
5Lyon laboratory, ANSES, Virology Unit, Lyon, France
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Séverine Murri
5Lyon laboratory, ANSES, Virology Unit, Lyon, France
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Coralie Pulido
6Lyon laboratory, ANSES, Platform of animal experiment, Lyon, France
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Johann Vulin
5Lyon laboratory, ANSES, Virology Unit, Lyon, France
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Francisco J Salguero
7Public Health England, Porton Down, UK
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Meriadeg Ar Gouilh
8Groupe de Recherche sur l’Adaptation Microbienne, Normandie Université, Caen, France
9Service de Virologie, CHU de Caen, Caen, France
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Alexandre Servat
3Nancy laboratory for rabies and wildlife, ANSES, Lyssavirus Unit, Malzéville, France
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Philippe Marianneau
5Lyon laboratory, ANSES, Virology Unit, Lyon, France
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Abstract

Understanding the pathogenesis of the SARS-CoV-2 infection is key to develop preventive and therapeutic strategies against COVID-19, in the case of severe illness but also when the disease is mild. The use of appropriate experimental animal models remains central in the in-vivo exploration of the physiopathology of infection and antiviral strategies. This study describes SARS-CoV-2 intra-nasal infection in ferrets and hamsters with low doses of low-passage SARS-CoV-2 clinical French isolate UCN19, describing infection levels, excretion, immune responses and pathological patterns in both animal species. Individual infection with 103 pfu SARS-CoV-2 induced a more severe disease in hamsters than in ferrets. Viral RNA was detected in the lungs of hamsters but not of ferrets and in the brain (olfactive and/or spinal bulbs) of both species. Overall, the clinical disease remained mild, with serological responses detected from 7 days and 10 days post inoculation in hamsters and ferrets respectively. Virus became undetectable and pathology resolved within 14 days. The kinetics and levels of infection can be used in ferrets and hamsters as experimental models for understanding the pathogenicity of SARS-CoV-2, and testing the protective effect of drugs.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 24, 2020.
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Hamster and ferret experimental infection with intranasal low dose of a single strain of SARS-CoV-2
Elodie Monchatre-Leroy, Sandrine Lesellier, Marine Wasniewski, Evelyne Picard-Meyer, Céline Richomme, Franck Boué, Sandra Lacôte, Séverine Murri, Coralie Pulido, Johann Vulin, Francisco J Salguero, Meriadeg Ar Gouilh, Alexandre Servat, Philippe Marianneau
bioRxiv 2020.09.24.311977; doi: https://doi.org/10.1101/2020.09.24.311977
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Hamster and ferret experimental infection with intranasal low dose of a single strain of SARS-CoV-2
Elodie Monchatre-Leroy, Sandrine Lesellier, Marine Wasniewski, Evelyne Picard-Meyer, Céline Richomme, Franck Boué, Sandra Lacôte, Séverine Murri, Coralie Pulido, Johann Vulin, Francisco J Salguero, Meriadeg Ar Gouilh, Alexandre Servat, Philippe Marianneau
bioRxiv 2020.09.24.311977; doi: https://doi.org/10.1101/2020.09.24.311977

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