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Mitochondrial alternative oxidase contributes to successful tardigrade anhydrobiosis

View ORCID ProfileDaria Wojciechowska, View ORCID ProfileAndonis Karachitos, View ORCID ProfileMilena Roszkowska, Wiktor Rzeźniczak, View ORCID ProfileRobert Sobkowiak, View ORCID ProfileŁukasz Kaczmarek, View ORCID ProfileJakub Kosicki, View ORCID ProfileHanna Kmita
doi: https://doi.org/10.1101/2020.09.25.313122
Daria Wojciechowska
1Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland
2Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
3Department of Animal Taxonomy and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4Department of Avian Biology and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
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  • ORCID record for Daria Wojciechowska
Andonis Karachitos
1Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland
2Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
3Department of Animal Taxonomy and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4Department of Avian Biology and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
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Milena Roszkowska
1Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland
2Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
3Department of Animal Taxonomy and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4Department of Avian Biology and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
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Wiktor Rzeźniczak
1Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland
2Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
3Department of Animal Taxonomy and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4Department of Avian Biology and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
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Robert Sobkowiak
1Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland
2Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
3Department of Animal Taxonomy and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4Department of Avian Biology and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
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Łukasz Kaczmarek
1Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland
2Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
3Department of Animal Taxonomy and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4Department of Avian Biology and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
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Jakub Kosicki
1Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland
2Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
3Department of Animal Taxonomy and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4Department of Avian Biology and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
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Hanna Kmita
1Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 2, 61-614 Poznań, Poland
2Department of Bioenergetics, Faculty of Biology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
3Department of Animal Taxonomy and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
4Department of Avian Biology and Ecology, Adam Mickiewicz University, Poznań, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
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  • For correspondence: kmita@amu.edu.pl
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ABSTRACT

Anhydrobiosis can be described as an adaptation to lack of water. This adaptation provides some organisms including tardigrades with a set of capabilities allowing them to survive extreme conditions that even do not exist on Earth. However, the underlying cellular mechanisms are still not explained. Available data assumes important contribution of mitochondrial proteins. Since mitochondrial alternative oxidase (AOX) described as a drought response element has recently been proposed for various invertebrates including tardigrades, we have decided to check if AOX is involved in successful anhydrobiosis of tardigrades. Milnesium inceptum was used as a model for the study. We confirmed functionality of M. inceptum AOX and estimated its activity contribution to anhydrobiosis of different duration. We observed that AOX activity was particularly important for M. inceptum revival after longer-term anhydrobiosis but did not affect rehydration stage. The results may contribute to explanation and then application of anhydrobiosis underlying mechanisms.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • In memory of Wiktor Rzeźniczak (1995-2020)

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted September 25, 2020.
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Mitochondrial alternative oxidase contributes to successful tardigrade anhydrobiosis
Daria Wojciechowska, Andonis Karachitos, Milena Roszkowska, Wiktor Rzeźniczak, Robert Sobkowiak, Łukasz Kaczmarek, Jakub Kosicki, Hanna Kmita
bioRxiv 2020.09.25.313122; doi: https://doi.org/10.1101/2020.09.25.313122
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Mitochondrial alternative oxidase contributes to successful tardigrade anhydrobiosis
Daria Wojciechowska, Andonis Karachitos, Milena Roszkowska, Wiktor Rzeźniczak, Robert Sobkowiak, Łukasz Kaczmarek, Jakub Kosicki, Hanna Kmita
bioRxiv 2020.09.25.313122; doi: https://doi.org/10.1101/2020.09.25.313122

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