Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Improved yellow-green split fluorescent proteins for protein labeling and signal amplification

View ORCID ProfileShuqin Zhou, View ORCID ProfileSiyu Feng, View ORCID ProfileDavid Brown, View ORCID ProfileBo Huang
doi: https://doi.org/10.1101/2020.09.27.315697
Shuqin Zhou
1Department of Bioengineering and Therapeutical Sciences, University of California, San Francisco, San Francisco, CA, USA
2School of Pharmacy, Tsinghua University, Beijing. China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Shuqin Zhou
Siyu Feng
3UC Berkeley – UCSF Joint Graduate Program in Bioengineering, University of California, San Francisco, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Siyu Feng
David Brown
4Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for David Brown
Bo Huang
4Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA
5Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA
6Chan Zuckerberg Biohub, San Francisco, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Bo Huang
  • For correspondence: bo.huang@ucsf.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

The flexibility and versatility of self-complementing split fluorescent proteins (FPs) have enabled a wide range of applications. In particular, the FP1-10/11 split system contains a small fragment that facilitates efficient generation of endogenous-tagged cell lines and animals as well as signal amplification using tandem FP11 tags. To improve the FP1-10/11 toolbox we previously developed, here we used a combination of directed evolution and rational design approaches, resulting in two mNeonGreen (mNG)-based split FPs (mNG3A1-10/11 and mNG3K1-10/11) and one mClover-based split FP (CloGFP1-10/11). mNG3A1-10/11 and mNG3K1-10/11 not only enhanced the complementation efficiency at low expression levels, but also allowed us to demonstrate signal amplification using tandem mNG211 fragments in mammalian cells.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted September 28, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Improved yellow-green split fluorescent proteins for protein labeling and signal amplification
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Improved yellow-green split fluorescent proteins for protein labeling and signal amplification
Shuqin Zhou, Siyu Feng, David Brown, Bo Huang
bioRxiv 2020.09.27.315697; doi: https://doi.org/10.1101/2020.09.27.315697
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Improved yellow-green split fluorescent proteins for protein labeling and signal amplification
Shuqin Zhou, Siyu Feng, David Brown, Bo Huang
bioRxiv 2020.09.27.315697; doi: https://doi.org/10.1101/2020.09.27.315697

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Bioengineering
Subject Areas
All Articles
  • Animal Behavior and Cognition (4228)
  • Biochemistry (9107)
  • Bioengineering (6751)
  • Bioinformatics (23944)
  • Biophysics (12089)
  • Cancer Biology (9495)
  • Cell Biology (13740)
  • Clinical Trials (138)
  • Developmental Biology (7616)
  • Ecology (11661)
  • Epidemiology (2066)
  • Evolutionary Biology (15479)
  • Genetics (10618)
  • Genomics (14296)
  • Immunology (9463)
  • Microbiology (22792)
  • Molecular Biology (9078)
  • Neuroscience (48889)
  • Paleontology (355)
  • Pathology (1479)
  • Pharmacology and Toxicology (2565)
  • Physiology (3823)
  • Plant Biology (8308)
  • Scientific Communication and Education (1467)
  • Synthetic Biology (2290)
  • Systems Biology (6172)
  • Zoology (1297)