Abstract
Although the cerebellum is now recognized as part of a long-range brain network that serves limbic functions and motivated behavior, knowledge of cerebello-limbic connectivity is limited, and nothing is known about how the cerebellum connects functionally to the nucleus accumbens (NAc). Here, we report that stimulation of cerebellar nuclei in mice of both sexes modulates spiking activity in both NAc core and medial shell with fast excitation and slower, less synchronized inhibition. Fast responses would be well poised to support rapid communication of information critical to the control of motivated behavior, whereas slower responses may be suggestive of a regulatory function, such as gain control. Tracing experiments to chart cerebellar nuclei-NAc pathways identified disynaptic pathways that recruit the ventral tegmental area (VTA) and intralaminar thalamus (Centromedial and Parafascicular nuclei) as intermediary nodes. Optogenetic activation of cerebellar axons in each of these nodes was sufficient to evoke responses in both NAc core and medial shell, albeit with distinct, node-dependent properties. These pathways and the functional connectivity they support could underlie the role of the cerebellum in motivated behavior.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
↵a These authors share equal authorship
↵b These authors share senior authorship
In this substantially revised version of the manuscript, we present analyses of NAc single units, rather than multi-unit neural activity (revised Figures 1 and 2). We also present new optophysiology experiments with photostimulation of channelrhodopsin-expressing axons in VTA and intralaminar thalamus (revised Figures 6 and 7) and a revised discussion.