AcrIIA22 is a novel anti-CRISPR that impairs SpyCas9 activity by relieving DNA torsion of target plasmids

Kevin J. Forsberg; Danica T. Schmidtke; Rachel Werther; Deanna Hausman; Barry L. Stoddard; Brett K. Kaiser; Harmit S. Malik

doi:10.1101/2020.09.28.317578

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AcrIIA22 is a novel anti-CRISPR that impairs SpyCas9 activity by relieving DNA torsion of target plasmids

View ORCID ProfileKevin J. Forsberg, View ORCID ProfileDanica T. Schmidtke, Rachel Werther, View ORCID ProfileDeanna Hausman, Barry L. Stoddard, Brett K. Kaiser, View ORCID ProfileHarmit S. Malik

doi: https://doi.org/10.1101/2020.09.28.317578

Kevin J. Forsberg

aDivision of Basic Sciences Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

bHoward Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

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ORCID record for Kevin J. Forsberg
For correspondence: kforsber@fredhutch.org

Danica T. Schmidtke

aDivision of Basic Sciences Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

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Rachel Werther

aDivision of Basic Sciences Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

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Deanna Hausman

aDivision of Basic Sciences Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

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Barry L. Stoddard

aDivision of Basic Sciences Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

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Brett K. Kaiser

aDivision of Basic Sciences Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

cDepartment of Biology, Seattle University, 901 12th Avenue, Seattle, WA 98122, USA

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Harmit S. Malik

aDivision of Basic Sciences Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

bHoward Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109 USA

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ORCID record for Harmit S. Malik

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Posted September 29, 2020.

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AcrIIA22 is a novel anti-CRISPR that impairs SpyCas9 activity by relieving DNA torsion of target plasmids

Kevin J. Forsberg, Danica T. Schmidtke, Rachel Werther, Deanna Hausman, Barry L. Stoddard, Brett K. Kaiser, Harmit S. Malik

bioRxiv 2020.09.28.317578; doi: https://doi.org/10.1101/2020.09.28.317578

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AcrIIA22 is a novel anti-CRISPR that impairs SpyCas9 activity by relieving DNA torsion of target plasmids

Kevin J. Forsberg, Danica T. Schmidtke, Rachel Werther, Deanna Hausman, Barry L. Stoddard, Brett K. Kaiser, Harmit S. Malik

bioRxiv 2020.09.28.317578; doi: https://doi.org/10.1101/2020.09.28.317578

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