Abstract
Hydrogen Deuterium Exchange Mass Spectrometry is a powerful monitor of protein intrinsic dynamics, yet the interpretation, visualization and cross-comparison of HDX-MS datasets is challenging. Here we present PyHDX, an open-source python package and web server, that batch-extracts the universal quantity Gibbs free energy at residue level over multiple protein conditions and homologues. ΔG values relate to protein normal modes and together provide a universal measure of protein flexibility.
Availability PyHDX source code is released under the MIT license and can be accessed on GitHub.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Copyright
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