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Deploying and in vitro gut model to assay the impact of a mannan-oligosaccharide prebiotic, Bio-MOS® on the Atlantic salmon (Salmo salar) gut microbiome

R. Kazlauskaite, View ORCID ProfileB. Cheaib, View ORCID ProfileJ. Humble, View ORCID ProfileC. Heys, View ORCID ProfileU. Ijaz, View ORCID ProfileS. Connelly, View ORCID ProfileW.T. Sloan, View ORCID ProfileJ. Russell, L. Martinez-Rubio, J. Sweetman, A. Kitts, View ORCID ProfileP. McGinnity, P. Lyons, View ORCID ProfileM. Llewellyn
doi: https://doi.org/10.1101/2020.10.07.328427
R. Kazlauskaite
1Institute of Behaviour, Animal Health and Comparative Medicine, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ
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  • For correspondence: r.kazlauskaite.1@research.gla.ac.uk
B. Cheaib
1Institute of Behaviour, Animal Health and Comparative Medicine, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ
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  • ORCID record for B. Cheaib
J. Humble
1Institute of Behaviour, Animal Health and Comparative Medicine, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ
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C. Heys
1Institute of Behaviour, Animal Health and Comparative Medicine, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ
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U. Ijaz
2School of Engineering, University of Glasgow G12 8QQ
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S. Connelly
2School of Engineering, University of Glasgow G12 8QQ
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W.T. Sloan
2School of Engineering, University of Glasgow G12 8QQ
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J. Russell
2School of Engineering, University of Glasgow G12 8QQ
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L. Martinez-Rubio
3Mowi ASA, Bergen, Norway
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J. Sweetman
4Alltech Aqua, Eindhoven, Netherlands
5Alltech, Lexington, United States
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A. Kitts
1Institute of Behaviour, Animal Health and Comparative Medicine, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ
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P. McGinnity
6School of Biological, Earth and Environmental Sciences, University College Cork, T23 N73K, Cork, Ireland
7Marine Institute, Foras na Mara, F28 PF65, Newport, Ireland
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P. Lyons
4Alltech Aqua, Eindhoven, Netherlands
5Alltech, Lexington, United States
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M. Llewellyn
1Institute of Behaviour, Animal Health and Comparative Medicine, Graham Kerr Building, University of Glasgow, Glasgow G12 8QQ
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Abstract

Mannose-oligosaccharide (MOS) pre-biotics are widely deployed in animal agriculture as immunomodulators as well as to enhance growth and gut health. Their mode of action is thought to be mediated through their impact on host microbial communities and associated metabolism. Bio-MOS is a commercially available pre-biotic currently used in the agri-feed industry. To assay Bio-MOS for potential use as a pre-biotic growth promotor in salmonid aquaculture, we modified an established Atlantic salmon in vitro gut model, SalmoSim, to evaluate its impact on host microbial communities. In biological triplicate, microbial communities were stabilised in SalmoSim followed by twenty-day exposure to the pre-biotic and then an eight day ‘wash out’ period. Exposure the MOS resulted in a significant increase in formate (p=0.001), propionate (p=0.037) and isovalerate (p=0.024) levels, correlated with increased abundances of several principally anaerobic microbial genera (Fusobacteria, Agarivorans, Pseudoalteromonas, Myroides). 16S rDNA sequencing confirmed a significant shift in microbial community composition in response to supplementation. In conjunction with previous in vivo studies linking enhanced VFA production alongside MOS supplementation to host growth and performance, our data suggest that Bio-MOS may be of value in salmonid production. Furthermore, our data highlight the potential role of in vitro gut models to augment in vivo trials of microbiome modulators.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted October 07, 2020.
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Deploying and in vitro gut model to assay the impact of a mannan-oligosaccharide prebiotic, Bio-MOS® on the Atlantic salmon (Salmo salar) gut microbiome
R. Kazlauskaite, B. Cheaib, J. Humble, C. Heys, U. Ijaz, S. Connelly, W.T. Sloan, J. Russell, L. Martinez-Rubio, J. Sweetman, A. Kitts, P. McGinnity, P. Lyons, M. Llewellyn
bioRxiv 2020.10.07.328427; doi: https://doi.org/10.1101/2020.10.07.328427
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Deploying and in vitro gut model to assay the impact of a mannan-oligosaccharide prebiotic, Bio-MOS® on the Atlantic salmon (Salmo salar) gut microbiome
R. Kazlauskaite, B. Cheaib, J. Humble, C. Heys, U. Ijaz, S. Connelly, W.T. Sloan, J. Russell, L. Martinez-Rubio, J. Sweetman, A. Kitts, P. McGinnity, P. Lyons, M. Llewellyn
bioRxiv 2020.10.07.328427; doi: https://doi.org/10.1101/2020.10.07.328427

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