Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

High contrast probe cleavage detection on porous silicon biosensors via quantum dot labeled DNA probes

Rabeb Layouni, Michael Dubrovsky, Mengdi Bao, Haejun Chung, Ke Du, Svetlana V. Boriskina, Sharon M. Weiss, Diedrik Vermeulen
doi: https://doi.org/10.1101/2020.10.07.330589
Rabeb Layouni
1Department of Chemical and Biomolecular Engineering and Department of Electrical Engineering and Computer Science, Vanderbilt University, Nashville, TN 37235, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael Dubrovsky
2SiPhox Inc., 325 Vassar Street, Cambridge, MA 02139, USA
3Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: mdubrov@mit.edu
Mengdi Bao
4Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, NY 14623, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Haejun Chung
3Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ke Du
4Department of Mechanical Engineering, Rochester Institute of Technology, Rochester, NY 14623, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Svetlana V. Boriskina
3Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sharon M. Weiss
1Department of Chemical and Biomolecular Engineering and Department of Electrical Engineering and Computer Science, Vanderbilt University, Nashville, TN 37235, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Diedrik Vermeulen
2SiPhox Inc., 325 Vassar Street, Cambridge, MA 02139, USA
3Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Using porous silicon (PSi) interferometer sensors, we show the first experimental implementation of the high contrast probe cleavage detection (HCPCD) mechanism. HCPCD makes use of dramatic optical signal amplification caused by cleavage of high-contrast nanoparticle labels on probes instead of the capture of low-index biological molecules. An approximately 2 nm reflectance peak shift was detected after cleavage of DNA-quantum dot probes from the PSi surface via exposure to a 12.5 nM DNase enzyme solution for 2 hrs. This signal change is 20 times greater than the resolution of the spectrometer used for the interferometric measurements, and the interferometric measurements agree with the interferometric response predicted by simulations and fluorescence measurements. These proof of principle experiments show a clear path to real-time, highly sensitive and inexpensive point-of-care readout for a broad range of biological diagnostic assays that generate signal via nucleic acid cleavage.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵* mike{at}siphox.com; sharon.weiss{at}vanderbilt.edu

  • small fixes to references

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted October 09, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
High contrast probe cleavage detection on porous silicon biosensors via quantum dot labeled DNA probes
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
High contrast probe cleavage detection on porous silicon biosensors via quantum dot labeled DNA probes
Rabeb Layouni, Michael Dubrovsky, Mengdi Bao, Haejun Chung, Ke Du, Svetlana V. Boriskina, Sharon M. Weiss, Diedrik Vermeulen
bioRxiv 2020.10.07.330589; doi: https://doi.org/10.1101/2020.10.07.330589
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
High contrast probe cleavage detection on porous silicon biosensors via quantum dot labeled DNA probes
Rabeb Layouni, Michael Dubrovsky, Mengdi Bao, Haejun Chung, Ke Du, Svetlana V. Boriskina, Sharon M. Weiss, Diedrik Vermeulen
bioRxiv 2020.10.07.330589; doi: https://doi.org/10.1101/2020.10.07.330589

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Bioengineering
Subject Areas
All Articles
  • Animal Behavior and Cognition (3514)
  • Biochemistry (7371)
  • Bioengineering (5347)
  • Bioinformatics (20329)
  • Biophysics (10048)
  • Cancer Biology (7782)
  • Cell Biology (11353)
  • Clinical Trials (138)
  • Developmental Biology (6454)
  • Ecology (9985)
  • Epidemiology (2065)
  • Evolutionary Biology (13361)
  • Genetics (9377)
  • Genomics (12616)
  • Immunology (7729)
  • Microbiology (19119)
  • Molecular Biology (7478)
  • Neuroscience (41163)
  • Paleontology (301)
  • Pathology (1235)
  • Pharmacology and Toxicology (2142)
  • Physiology (3183)
  • Plant Biology (6885)
  • Scientific Communication and Education (1276)
  • Synthetic Biology (1900)
  • Systems Biology (5329)
  • Zoology (1091)