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Postharvest handling induces changes in fruit DNA methylation status and is associated with alterations in fruit quality in tomato (Solanum lycopersicum L.)

Jiaqi Zhou, Bixuan Chen, Karin Albornoz, Diane M Beckles
doi: https://doi.org/10.1101/2020.10.08.331819
Jiaqi Zhou
1Department of Plant Sciences, University of California, Davis, CA 95616, USA
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Bixuan Chen
1Department of Plant Sciences, University of California, Davis, CA 95616, USA
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Karin Albornoz
1Department of Plant Sciences, University of California, Davis, CA 95616, USA
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Diane M Beckles
1Department of Plant Sciences, University of California, Davis, CA 95616, USA
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  • For correspondence: dmbeckles@ucdavis.edu
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Abstract

Postharvest handling of tomato (Solanum lycopersicum L.), specifically low-temperature storage and early harvest are used to extend shelf life, but often reduce fruit quality. Recent work suggests that DNA methylation dynamics influences fruit ripening through the demethylase SlDML2 gene. However, the influence of postharvest handling on DNA methylation in relation to fruit quality is unclear. This work aimed to clarify these issues by analyzing DNA methylation using methyl-sensitive amplification polymorphism (MSAP), semi-quantitative transcriptional analysis of marker genes for fruit quality (RIN; RIPENING INHIBITOR) and DNA methylation (SlDML2; Solanum lycopersicum L. DNA demethylase 2), and, fruit biochemical quality biomarkers. Multivariate analysis of these data supported the view that DNA methylation of fruit was influenced more by postharvest handling than ripening stage, however, fruit quality was influenced mainly by ripening. Fruit chilled postharvest were distinct in their DNA methylation state and quality characteristics, which implied that these three phenomena i.e., chilling, methylation, and quality are highly connected. In addition, different postharvest handling methods modulated SlDML2 transcript levels but had little effect on the level of RIN transcripts in fruit that reached the Turning stage after early harvest, and cold storage. These data collectively helped to advance our interpretation of tomato fruit ripening. In conclusion, our findings revealed that postharvest-induced variation in fruit quality is in relation to DNA methylation. Long-term this work will help better connect physiological changes in tomato fruit to events happening at the molecular level.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵! Departamento de Produccion Vegetal, Universidad de Concepcion, Region del BioBio, Chile

  • Abbreviations: MSAP, methyl-sensitive amplification polymorphism; ‘FH’, fresh-harvested fruit; ‘M’, Mature green; ‘T’, Turning; ‘5M’, fruit harvested at Mature green and stored at 5°C after 14 days; ‘5T’, ‘5M’ fruit stored at 20°C until Turning; ‘20T’ and ‘12.5T’, fruit early harvested at Mature green and stored at 20°C and 12.5°C, respectively; SRT-PCR, Semiquantitative Reverse Transcription-Polymerase Chain Reaction; RT-qPCR, Quantitative real-time PCR; TA, titratable acidity; SlDML2, Solanum lycopersicum L. DNA demethylase 2; RIN, RIPENING INHIBITOR.

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Posted October 09, 2020.
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Postharvest handling induces changes in fruit DNA methylation status and is associated with alterations in fruit quality in tomato (Solanum lycopersicum L.)
Jiaqi Zhou, Bixuan Chen, Karin Albornoz, Diane M Beckles
bioRxiv 2020.10.08.331819; doi: https://doi.org/10.1101/2020.10.08.331819
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Postharvest handling induces changes in fruit DNA methylation status and is associated with alterations in fruit quality in tomato (Solanum lycopersicum L.)
Jiaqi Zhou, Bixuan Chen, Karin Albornoz, Diane M Beckles
bioRxiv 2020.10.08.331819; doi: https://doi.org/10.1101/2020.10.08.331819

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