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Screening of tomato seed bacterial endophytes for antifungal activity reveals lipopeptide producing Bacillus siamensis as a potential bio-control agent

Ayushi Sharma, Nutan Kaushik, Abhishek Sharma, Abhay Bajaj, Mandar Rasane, Yogesh S. Shouche, Takwa Marzouk, Naceur Djébali
doi: https://doi.org/10.1101/2020.10.09.332627
Ayushi Sharma
1Amity Food and Agriculture Foundation, Amity University Uttar Pradesh, Noida-201313, India
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Nutan Kaushik
1Amity Food and Agriculture Foundation, Amity University Uttar Pradesh, Noida-201313, India
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  • For correspondence: kaushikn2008@gmail.com nkaushik5@amity.edu
Abhishek Sharma
1Amity Food and Agriculture Foundation, Amity University Uttar Pradesh, Noida-201313, India
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Abhay Bajaj
2National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, India
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Mandar Rasane
2National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, India
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Yogesh S. Shouche
2National Centre for Cell Science, NCCS Complex, Savitribai Phule Pune University Campus, India
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Takwa Marzouk
3Centre of Biotechnology of Borj Cedria (CBBC), Laboratory of Bioactive Substances, BP 901 Hammam-lif 2050, Tunisia
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Naceur Djébali
3Centre of Biotechnology of Borj Cedria (CBBC), Laboratory of Bioactive Substances, BP 901 Hammam-lif 2050, Tunisia
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ABSTRACT

The current study investigates the diversity pattern and fungicidal potential of bacterial endophytes isolated from two different organic varieties of tomato plants (V1 and V2). A total of seventy-four bacterial isolates identified by 16S rRNA sequencing revealed a single genus Bacillus with 16 different species. The Shannon diversity H’ (1.45), Simpson’s index of diversity (0.9), Magalef’ index (2.1), Evenness (0.96), and Species richness (8) indicated the high endophytic bacterial diversity in the V1 variety of the tomato. Bacterial endophytes isolated from both the varieties were screened for their antifungal activity against five economically critical fungal pathogens (viz., Botrytis cinerea, Rhizoctonia solani, Fusarium solani, Verticillium lateritium, and Alternaria solani) of tomato crop through dual culture assay. The data revealed B. siamensis KCTC 13613(T) as the most potent antagonist significantly (p < 0.05), inhibiting the mycelial growth between 75 to 90% against selected fungal pathogens. High bioactivity of lipopeptide extract of B. siamensis was recorded against R. solani with IC50 value of 72 ppm. The UPLC-HDMS analysis of this lipopeptide extract revealed the presence of, Surfactin and Bacillomycin D.

Competing Interest Statement

The authors have declared no competing interest.

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Posted October 09, 2020.
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Screening of tomato seed bacterial endophytes for antifungal activity reveals lipopeptide producing Bacillus siamensis as a potential bio-control agent
Ayushi Sharma, Nutan Kaushik, Abhishek Sharma, Abhay Bajaj, Mandar Rasane, Yogesh S. Shouche, Takwa Marzouk, Naceur Djébali
bioRxiv 2020.10.09.332627; doi: https://doi.org/10.1101/2020.10.09.332627
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Screening of tomato seed bacterial endophytes for antifungal activity reveals lipopeptide producing Bacillus siamensis as a potential bio-control agent
Ayushi Sharma, Nutan Kaushik, Abhishek Sharma, Abhay Bajaj, Mandar Rasane, Yogesh S. Shouche, Takwa Marzouk, Naceur Djébali
bioRxiv 2020.10.09.332627; doi: https://doi.org/10.1101/2020.10.09.332627

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