Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A 180 My-old female-specific genome region in sturgeon reveals the oldest known vertebrate sex determining system with undifferentiated sex chromosomes

View ORCID ProfileHeiner Kuhl, View ORCID ProfileYann Guiguen, Christin Höhne, Eva Kreuz, Kang Du, Christophe Klopp, Céline Lopez-Roques, Elena Santidrian Yebra-Pimentel, Mitica Ciorpac, Jörn Gessner, Daniela Holostenco, Wibke Kleiner, View ORCID ProfileKlaus Kohlmann, View ORCID ProfileDunja K. Lamatsch, Dmitry Prokopov, Anastasia Bestin, Emmanuel Bonpunt, Bastien Debeuf, Pierrick Haffray, Romain Morvezen, Pierre Patrice, Radu Suciu, Ron Dirks, View ORCID ProfileSven Wuertz, View ORCID ProfileWerner Kloas, View ORCID ProfileManfred Schartl, View ORCID ProfileMatthias Stöck
doi: https://doi.org/10.1101/2020.10.10.334367
Heiner Kuhl
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Heiner Kuhl
  • For correspondence: kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
Yann Guiguen
2INRAE, LPGP, 35000, Rennes, France., e-mail:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yann Guiguen
  • For correspondence: yann.guiguen@inrae.fr
Christin Höhne
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
Eva Kreuz
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
Kang Du
3Developmental Biochemistry, Biocenter, University of Wuerzburg, Wuerzburg, Germany, email: ,
14The Xiphophorus Genetic Stock Center, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dukang1117@outlook.com phch1@biozentrum.uni-wuerzburg.de
Christophe Klopp
4SIGENAE, Plate-forme Bio-informatique Genotoul, Mathématiques et Informatique Appliquées de Toulouse, INRAe, Castanet-Tolosan, France, email:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: christophe.klopp@inrae.fr
Céline Lopez-Roques
5INRA, US 1426, GeT-PlaGe, Genotoul, Castanet-Tolosan, France, e-mail:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: celine.lopez-roques@inrae.fr
Elena Santidrian Yebra-Pimentel
6Future Genomics Technologies B.V., Sylviusweg 74, 2333 BD Leiden, The Netherlands, e-mails: ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dirks@futuregenomics.tech santidrian@futuregenomics.tech
Mitica Ciorpac
7Danube Delta National Institute for Research and Development, Tulcea 820112, Romania, e-mail: ,
8Genetic Improvement Laboratory, Research Station for Cattle Breeding Dancu - Iasi (SCDCB Dancu), Academy of Agricultural and Forestry Sciences “Gheorghe Ionescu-Sisesti”, Romania (Mail address: Iasi-Ungheni Street, No. 9, Holboca, Iași county, 707252, Romania), e-mail:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dana.holostenco@gmail.com radu.suciu34@gmail.com ciorpac.mitica@gmail.com
Jörn Gessner
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
Daniela Holostenco
7Danube Delta National Institute for Research and Development, Tulcea 820112, Romania, e-mail: ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dana.holostenco@gmail.com radu.suciu34@gmail.com
Wibke Kleiner
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
Klaus Kohlmann
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Klaus Kohlmann
  • For correspondence: kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
Dunja K. Lamatsch
9University of Innsbruck, Research Department for Limnology, Mondsee, Austria, e-mail:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Dunja K. Lamatsch
  • For correspondence: dunja.lamatsch@uibk.ac.at
Dmitry Prokopov
10Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia, e-mail:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dprokopov@mcb.nsc.ru
Anastasia Bestin
11SYSAAF, Station INRAE-LPGP, Campus de Beaulieu, 35042, Rennes cedex, France, e-mails: , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: anastasia.bestin@inrae.fr pierrick.haffray@inrae.fr Romain.Morvezen@inrae.fr Pierre.Patrice@inrae.fr
Emmanuel Bonpunt
12L’Esturgeonnière, Allée de Mios, 33470 Le Teich, France, e-mail:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: emmanuelb@caviar-perlita.com
Bastien Debeuf
13SCEA Sturgeon, 29 rue du Carillon, 17240 Saint Fort sur Gironde, France, e-mail:
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: b.debeuf@kaviar.com
Pierrick Haffray
11SYSAAF, Station INRAE-LPGP, Campus de Beaulieu, 35042, Rennes cedex, France, e-mails: , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: anastasia.bestin@inrae.fr pierrick.haffray@inrae.fr Romain.Morvezen@inrae.fr Pierre.Patrice@inrae.fr
Romain Morvezen
11SYSAAF, Station INRAE-LPGP, Campus de Beaulieu, 35042, Rennes cedex, France, e-mails: , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: anastasia.bestin@inrae.fr pierrick.haffray@inrae.fr Romain.Morvezen@inrae.fr Pierre.Patrice@inrae.fr
Pierre Patrice
11SYSAAF, Station INRAE-LPGP, Campus de Beaulieu, 35042, Rennes cedex, France, e-mails: , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: anastasia.bestin@inrae.fr pierrick.haffray@inrae.fr Romain.Morvezen@inrae.fr Pierre.Patrice@inrae.fr
Radu Suciu
7Danube Delta National Institute for Research and Development, Tulcea 820112, Romania, e-mail: ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dana.holostenco@gmail.com radu.suciu34@gmail.com
Ron Dirks
6Future Genomics Technologies B.V., Sylviusweg 74, 2333 BD Leiden, The Netherlands, e-mails: ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dirks@futuregenomics.tech santidrian@futuregenomics.tech
Sven Wuertz
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sven Wuertz
  • For correspondence: kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
Werner Kloas
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Werner Kloas
  • For correspondence: kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
Manfred Schartl
3Developmental Biochemistry, Biocenter, University of Wuerzburg, Wuerzburg, Germany, email: ,
14The Xiphophorus Genetic Stock Center, Department of Chemistry and Biochemistry, Texas State University, San Marcos, TX, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Manfred Schartl
  • For correspondence: dukang1117@outlook.com phch1@biozentrum.uni-wuerzburg.de
Matthias Stöck
1Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Department of Ecophysiology and Aquaculture, Müggelseedamm 301 & 310, D-12587 Berlin, Germany, e-mails: , , , , , , ,
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Matthias Stöck
  • For correspondence: matthias.stoeck@igb-berlin.de kuhl@igb-berlin.de hoehne@igb-berlin.de kreuz@igb-berlin.de sturgeon@igb-berlin.de werner.kloas@igb-berlin.de wibke.kleiner@igb-berlin.de kohlmann@igb-berlin.de wuertz@igb-berlin.de
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Summary

Several hypotheses explain the prevalence of undifferentiated sex chromosomes in poikilothermic vertebrates. Turnovers change the master sex determination gene, the sex chromosome or the sex determination system (e.g. XY to WZ). Jumping master genes stay main triggers but translocate to other chromosomes. Occasional recombination (e.g. in sex-reversed females) prevents sex chromosome degeneration. Recent research has uncovered conserved heteromorphic or even homomorphic sex chromosomes in several clades of non-avian and non-mammalian vertebrates. Sex determination in sturgeons (Acipenseridae) has been a long-standing basic biological question, linked to economical demands by the caviar-producing aquaculture. Here, we report the discovery of a sex-specific sequence from sterlet (Acipenser ruthenus). Using chromosome-scale assemblies and pool-sequencing, we first identified a ~16 kb female-specific region. We developed a PCR-genotyping test, yielding female-specific products in six species, spanning the entire phylogeny with the most divergent extant lineages (A. sturio, A. oxyrinchus vs. A. ruthenus, Huso huso), stemming from an ancient tetraploidization. Similar results were obtained in two octoploid species (A. gueldenstaedtii, A. baerii). Conservation of a female-specific sequence for a long period, representing 180 My of sturgeon evolution, and across at least one polyploidization event, raises many interesting biological questions. We discuss a conserved undifferentiated sex chromosome system with a ZZ/ZW-mode of sex determination and potential alternatives.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • minor revisions

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted October 22, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A 180 My-old female-specific genome region in sturgeon reveals the oldest known vertebrate sex determining system with undifferentiated sex chromosomes
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A 180 My-old female-specific genome region in sturgeon reveals the oldest known vertebrate sex determining system with undifferentiated sex chromosomes
Heiner Kuhl, Yann Guiguen, Christin Höhne, Eva Kreuz, Kang Du, Christophe Klopp, Céline Lopez-Roques, Elena Santidrian Yebra-Pimentel, Mitica Ciorpac, Jörn Gessner, Daniela Holostenco, Wibke Kleiner, Klaus Kohlmann, Dunja K. Lamatsch, Dmitry Prokopov, Anastasia Bestin, Emmanuel Bonpunt, Bastien Debeuf, Pierrick Haffray, Romain Morvezen, Pierre Patrice, Radu Suciu, Ron Dirks, Sven Wuertz, Werner Kloas, Manfred Schartl, Matthias Stöck
bioRxiv 2020.10.10.334367; doi: https://doi.org/10.1101/2020.10.10.334367
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
A 180 My-old female-specific genome region in sturgeon reveals the oldest known vertebrate sex determining system with undifferentiated sex chromosomes
Heiner Kuhl, Yann Guiguen, Christin Höhne, Eva Kreuz, Kang Du, Christophe Klopp, Céline Lopez-Roques, Elena Santidrian Yebra-Pimentel, Mitica Ciorpac, Jörn Gessner, Daniela Holostenco, Wibke Kleiner, Klaus Kohlmann, Dunja K. Lamatsch, Dmitry Prokopov, Anastasia Bestin, Emmanuel Bonpunt, Bastien Debeuf, Pierrick Haffray, Romain Morvezen, Pierre Patrice, Radu Suciu, Ron Dirks, Sven Wuertz, Werner Kloas, Manfred Schartl, Matthias Stöck
bioRxiv 2020.10.10.334367; doi: https://doi.org/10.1101/2020.10.10.334367

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Evolutionary Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4078)
  • Biochemistry (8750)
  • Bioengineering (6467)
  • Bioinformatics (23314)
  • Biophysics (11719)
  • Cancer Biology (9134)
  • Cell Biology (13227)
  • Clinical Trials (138)
  • Developmental Biology (7404)
  • Ecology (11360)
  • Epidemiology (2066)
  • Evolutionary Biology (15078)
  • Genetics (10390)
  • Genomics (14001)
  • Immunology (9109)
  • Microbiology (22025)
  • Molecular Biology (8773)
  • Neuroscience (47315)
  • Paleontology (350)
  • Pathology (1419)
  • Pharmacology and Toxicology (2480)
  • Physiology (3701)
  • Plant Biology (8044)
  • Scientific Communication and Education (1427)
  • Synthetic Biology (2206)
  • Systems Biology (6009)
  • Zoology (1247)