Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A single-cell atlas of Plasmodium falciparum transmission through the mosquito

View ORCID ProfileEliana Real, View ORCID ProfileVirginia M. Howick, View ORCID ProfileFarah Dahalan, View ORCID ProfileKathrin Witmer, View ORCID ProfileJuliana Cudini, View ORCID ProfileClare Andradi-Brown, View ORCID ProfileJoshua Blight, Mira S. Davidson, View ORCID ProfileSunil Kumar Dogga, View ORCID ProfileAdam J. Reid, View ORCID ProfileJake Baum, View ORCID ProfileMara K. N. Lawniczak
doi: https://doi.org/10.1101/2020.10.11.333179
Eliana Real
1Department of Life Sciences, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Eliana Real
Virginia M. Howick
2Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK
3Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
4Wellcome Centre for Integrative Parasitology, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Virginia M. Howick
Farah Dahalan
1Department of Life Sciences, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Farah Dahalan
Kathrin Witmer
1Department of Life Sciences, Imperial College London, London, UK
2Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kathrin Witmer
Juliana Cudini
2Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Juliana Cudini
Clare Andradi-Brown
1Department of Life Sciences, Imperial College London, London, UK
5Department of Infectious Disease, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Clare Andradi-Brown
Joshua Blight
1Department of Life Sciences, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Joshua Blight
Mira S. Davidson
1Department of Life Sciences, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sunil Kumar Dogga
2Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sunil Kumar Dogga
Adam J. Reid
2Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Adam J. Reid
Jake Baum
1Department of Life Sciences, Imperial College London, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jake Baum
  • For correspondence: jake.baum@imperial.ac.uk mara@sanger.ac.uk
Mara K. N. Lawniczak
2Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mara K. N. Lawniczak
  • For correspondence: jake.baum@imperial.ac.uk mara@sanger.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Data/Code
  • Preview PDF
Loading

Abstract

Malaria parasites have a complex life cycle featuring diverse developmental strategies, each uniquely adapted to navigate specific host environments. Here we use single-cell transcriptomics to illuminate gene usage across the transmission cycle of the most virulent agent of human malaria – Plasmodium falciparum. We reveal developmental trajectories associated with the colonisation of the mosquito midgut and salivary glands and elucidate the transcriptional signatures of each transmissible stage. Additionally, we identify both conserved and nonconserved gene usage between human and rodent parasites, which point to both essential mechanisms in malaria transmission and species-specific adaptations potentially linked to host tropism. Together, the data presented here, which are made freely available via an interactive website, establish the most complete atlas of the P. falciparum transcriptional journey to date.

One sentence summary Single-cell transcriptomics of P. falciparum transmission stages highlights developmental trajectories and gene usage.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • http://malariacellatlas.org/

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
Back to top
PreviousNext
Posted October 11, 2020.
Download PDF

Supplementary Material

Data/Code
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A single-cell atlas of Plasmodium falciparum transmission through the mosquito
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A single-cell atlas of Plasmodium falciparum transmission through the mosquito
Eliana Real, Virginia M. Howick, Farah Dahalan, Kathrin Witmer, Juliana Cudini, Clare Andradi-Brown, Joshua Blight, Mira S. Davidson, Sunil Kumar Dogga, Adam J. Reid, Jake Baum, Mara K. N. Lawniczak
bioRxiv 2020.10.11.333179; doi: https://doi.org/10.1101/2020.10.11.333179
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
A single-cell atlas of Plasmodium falciparum transmission through the mosquito
Eliana Real, Virginia M. Howick, Farah Dahalan, Kathrin Witmer, Juliana Cudini, Clare Andradi-Brown, Joshua Blight, Mira S. Davidson, Sunil Kumar Dogga, Adam J. Reid, Jake Baum, Mara K. N. Lawniczak
bioRxiv 2020.10.11.333179; doi: https://doi.org/10.1101/2020.10.11.333179

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Genomics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3601)
  • Biochemistry (7567)
  • Bioengineering (5521)
  • Bioinformatics (20782)
  • Biophysics (10325)
  • Cancer Biology (7978)
  • Cell Biology (11634)
  • Clinical Trials (138)
  • Developmental Biology (6602)
  • Ecology (10200)
  • Epidemiology (2065)
  • Evolutionary Biology (13610)
  • Genetics (9539)
  • Genomics (12844)
  • Immunology (7919)
  • Microbiology (19538)
  • Molecular Biology (7657)
  • Neuroscience (42080)
  • Paleontology (308)
  • Pathology (1257)
  • Pharmacology and Toxicology (2201)
  • Physiology (3267)
  • Plant Biology (7038)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1951)
  • Systems Biology (5426)
  • Zoology (1116)