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Differential gene expression in the interactome of the Human Dopamine transporter in the context of Parkinson’s disease

View ORCID ProfileRoberto Navarro Quiroz, View ORCID ProfileAna Ligia Scott, View ORCID ProfileEric Allison Philot, View ORCID ProfileLinda Atencio, View ORCID ProfileCecilia Fernandez Ponce, View ORCID ProfileGustavo Aroca Martinez, View ORCID ProfileAndres Cadena Bonfanti, View ORCID ProfileLorena Gomez Escorcia, View ORCID ProfileElkin Navarro Quiroz
doi: https://doi.org/10.1101/2020.10.12.336388
Roberto Navarro Quiroz
1CMCC—Centro de Matemática, Computação e Cognição, Laboratório do Biología Computacional e Bioinformática—LBCB, Universidade Federal do ABC, Sao Paulo 01023, Brazil
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  • For correspondence: robertcnavarro@gmail.com
Ana Ligia Scott
1CMCC—Centro de Matemática, Computação e Cognição, Laboratório do Biología Computacional e Bioinformática—LBCB, Universidade Federal do ABC, Sao Paulo 01023, Brazil
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Eric Allison Philot
1CMCC—Centro de Matemática, Computação e Cognição, Laboratório do Biología Computacional e Bioinformática—LBCB, Universidade Federal do ABC, Sao Paulo 01023, Brazil
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Linda Atencio
2Universidad Simón Bolívar, School of Medicine, Barranquilla, Colombia
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Cecilia Fernandez Ponce
3Universidad de Cadiz, Department of Biomedicine, Biotechnology and Public Health, Cadiz, Spain
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Gustavo Aroca Martinez
4Clínica de la Costa, Barranquilla, Colombia
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Andres Cadena Bonfanti
4Clínica de la Costa, Barranquilla, Colombia
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Lorena Gomez Escorcia
2Universidad Simón Bolívar, School of Medicine, Barranquilla, Colombia
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Elkin Navarro Quiroz
2Universidad Simón Bolívar, School of Medicine, Barranquilla, Colombia
5Fundación Universitaria San Martin, School of Medicine, Puerto Colombia, Colombia
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Abstract

Background The human dopamine transporter is the main regulator of dopamine tone and an intricate network exists to regulate the expression, conformation, and kinetics of the hDAT. hDAT dysfunction is directly related to Parkinson’s disease. The objective of this work is to evaluate the differential gene expression in the interactome of the Dopamine transporter in the context of Parkinson’s disease.

Methods To do this, we evaluated hDAT interaction data in string-db, mint.bio, IntAct, reactome, hprd and BioGRID, subsequently, data was obtained from the differential gene expression of mRNA and miRNAs for this hDAT interactome in the context of PD.

Results The analysis of the differential expression changes of genes of the hDAT interactome in tissues of patients with PD compared with tissues of individuals without PD, allowed to identify an expression pattern of 32 components of the hDAT interactome, of which 31 presented a negative change proportion in PD.

Conclusions We found a total of 90 miRNAs that could regulate the expression of 27 components of the hDAT interactome, at the same time, 39 components of the hDAT interactome may participate in 40 metabolic pathways. Together, these findings show a systematic effect on the hDAT-mediated dopamine internalization process in patients with Parkinson’s, which would contribute to a greater susceptibility to neuronal oxidative stress in PD patients.

Competing Interest Statement

The authors have declared no competing interest.

  • List of abbreviations

    CAMK2A
    CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE II-ALPHA; CAMK2A
    CSK
    CYTOPLASMIC TYROSINE KINASE
    EPN1
    EPS-15-Interacting Protein 1
    EPS15
    Epsin-1
    MTNR1
    MELATONIN RECEPTOR 1A
    NEDD4
    NEURAL PRECURSOR CELL EXPRESSED, DEVELOPMENTALLY DOWNREGULATED 4
    GEO
    the Genetic Expression Omnibus PARK2 E3 ubiquitin-protein ligase parkin
    PICK1
    PRKCA-binding protein
    hDAT
    The human dopamine transporter
    TPPP
    TUBULIN POLYMERIZATION-PROMOTING PROTEIN
    SLC6A3
    SOLUTE CARRIER FAMILY 6 (NEUROTRANSMITTER TRANSPORTER, DOPAMINE), MEMBER 3
  • Copyright 
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    Posted October 12, 2020.
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    Differential gene expression in the interactome of the Human Dopamine transporter in the context of Parkinson’s disease
    Roberto Navarro Quiroz, Ana Ligia Scott, Eric Allison Philot, Linda Atencio, Cecilia Fernandez Ponce, Gustavo Aroca Martinez, Andres Cadena Bonfanti, Lorena Gomez Escorcia, Elkin Navarro Quiroz
    bioRxiv 2020.10.12.336388; doi: https://doi.org/10.1101/2020.10.12.336388
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    Differential gene expression in the interactome of the Human Dopamine transporter in the context of Parkinson’s disease
    Roberto Navarro Quiroz, Ana Ligia Scott, Eric Allison Philot, Linda Atencio, Cecilia Fernandez Ponce, Gustavo Aroca Martinez, Andres Cadena Bonfanti, Lorena Gomez Escorcia, Elkin Navarro Quiroz
    bioRxiv 2020.10.12.336388; doi: https://doi.org/10.1101/2020.10.12.336388

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