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The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast fusion of the readily releasable pool of vesicles

View ORCID ProfileChad Paul Grabner, View ORCID ProfileTobias Moser
doi: https://doi.org/10.1101/2020.10.12.336503
Chad Paul Grabner
1Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany
2Synaptic Nanophysiology Group, Max Planck Institute of Biophysical Chemistry, 37077 Göttingen, Germany
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  • For correspondence: chadgrabner@gmail.com
Tobias Moser
1Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, Germany
2Synaptic Nanophysiology Group, Max Planck Institute of Biophysical Chemistry, 37077 Göttingen, Germany
3Collaborative Research Center 889, University of Göttingen, Göttingen, Germany
4Multiscale Bioimaging Cluster of Excellence (MBExC), University of Göttingen, 37075 Göttingen, Germany
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Abstract

Rod photoreceptors (PRs) use ribbon synapses to transmit visual information. To signal ‘no light detected’ they release glutamate continually to activate post-synaptic receptors, and when light is detected glutamate release pauses. How a rod’s individual ribbon enables this process was studied here by recording evoked changes in whole-cell membrane capacitance from wild type and ribbonless (RIBEYE-ko) rods. Wild type rods created a readily releasable pool (RRP) of 92 synaptic vesicles (SVs) that emptied as a single kinetic phase with a τ < 0.4 msec. Lowering intracellular Ca2+-buffering accelerated Cav channel opening and facilitated release kinetics, but RRP size was unaltered. In contrast, ribbonless rods created an RRP of 24 SVs, and lacked Cav channel facilitation; however, Ca2+ channel-release coupling remained tight. The release deficits caused a sharp attenuation of rod-driven light responses measured from RIBEYE-ko mice. We conclude that the synaptic ribbon facilitates Ca2+-influx and establishes a large RRP of SVs.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 12, 2020.
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The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast fusion of the readily releasable pool of vesicles
Chad Paul Grabner, Tobias Moser
bioRxiv 2020.10.12.336503; doi: https://doi.org/10.1101/2020.10.12.336503
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The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast fusion of the readily releasable pool of vesicles
Chad Paul Grabner, Tobias Moser
bioRxiv 2020.10.12.336503; doi: https://doi.org/10.1101/2020.10.12.336503

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