ABSTRACT
The human transient receptor potential vanilloid 4 (hTRPV4) ion channel plays a critical role in a variety of biological processes. Whilst the activation of hTRPV4 gating properties has been reported for a broad spectrum of stimuli, including synthetic 4α-phorbols, the molecular basis of the activation is poorly understood. Here we report the novel cryo-EM structure of the hTRPV4 determined in the presence of the archetypical phorbol acid agonist, 4α-PDD. Complementary mutagenesis experiments support the EM-identified binding site as well as allowing rationalization of disruptive mutants located outside of the 4α-PDD binding site. This work represents the first structural information of hTRPV4 in a ligand-induced open conformation. Together, our data reveal the underlying molecular mechanisms resulting in the opening of the central pore and ion-channel activation and provide a structural template for designing inhibitors targeting the open-state conformation of hTRPV4.
Competing Interest Statement
The authors declare the following competing financial interest(s): A.K.C.U., U.E., D.G., V.P., U.E-K., A.B., A.B. and S.J.H. are / were employees of Bayer AG and may have additional stock options. M.B., N.B., D.B. and M.H. are employees of leadXpro AG and may have additional stock options. The other authors declare that no competing interests exist.
