Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Evaluation of the antiviral effect of chlorine dioxide (ClO2) using a vertebrate model inoculated with avian coronavirus

Xóchitl Zambrano-Estrada, Carlos Domínguez-Sánchez, Marina Banuet-Martínez, Fabiola Guerrero de la Rosa, View ORCID ProfileTeresa García-Gasca, Luis Prieto-Valiente, View ORCID ProfileKarina Acevedo-Whitehouse
doi: https://doi.org/10.1101/2020.10.13.336768
Xóchitl Zambrano-Estrada
aLaboratory of Veterinary Diagnostic Pathology. School of Natural Sciences. Autonomous University of Queretaro. Av. De las Ciencias S/N. Juriquilla, Queretaro. Qro. C.P.76230. Mexico
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carlos Domínguez-Sánchez
bUnit for Basic and Applied Microbiology. School of Natural Sciences. Autonomous University of Queretaro. Anillo Vial Fray Junípero Serra, Querétaro, Qro. C.P. 76140, Mexico
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marina Banuet-Martínez
bUnit for Basic and Applied Microbiology. School of Natural Sciences. Autonomous University of Queretaro. Anillo Vial Fray Junípero Serra, Querétaro, Qro. C.P. 76140, Mexico
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fabiola Guerrero de la Rosa
bUnit for Basic and Applied Microbiology. School of Natural Sciences. Autonomous University of Queretaro. Anillo Vial Fray Junípero Serra, Querétaro, Qro. C.P. 76140, Mexico
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Teresa García-Gasca
cLaboratory of Cellular Biology. School of Natural Sciences. Autonomous University of Queretaro Av. De las Ciencias S/N. Juriquilla, Queretaro. Qro. C.P. 76230. Mexico
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Teresa García-Gasca
Luis Prieto-Valiente
dBioestadística Médica y Metodología de la Investigación, Análisis Estadístico y Big Data, Universidad Católica de Murcia. Av. de los Jerónimos, 135 Guadalupe de Maciascoque Murcia, 30107. Spain
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karina Acevedo-Whitehouse
bUnit for Basic and Applied Microbiology. School of Natural Sciences. Autonomous University of Queretaro. Anillo Vial Fray Junípero Serra, Querétaro, Qro. C.P. 76140, Mexico
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Karina Acevedo-Whitehouse
  • For correspondence: karina.acevedo.whitehouse@uaq.mx
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Background The need for safe and effective antiviral treatments is pressing given the number of viral infections that are prevalent in animal and human populations, often causing devastating economic losses and mortality. Informal accounts of anecdotal use of chlorine dioxide (ClO2), a well-known disinfectant and antiseptic, in COVID-19 patients has raised concern about potential toxicity, but also raises the question that ClO2 might elicit antiviral effects, a possibility that has never been examined in vivo in any animal model. Here, we challenged the hypothesis that ClO2 decreases the viral load and virus-induced mortality in a vertebrate model. For this, we determined viral load, virus-induced lesions and mortality in 10-day old chick embryos inoculated with 104 mean EID50/mL of attenuated Massachusetts and Connecticut avian coronavirus (IBV) strains.

Results The ClO2 treatment had a marked impact on IBV infection. Namely, viral titres were 2.4-fold lower and mortality was reduced by half in infected embryos that were treated with ClO2. Infection led to developmental abnormalities regardless of treatment. Lesions typical of IBV infections were observed in all inoculated embryos, but severity tended to be significantly lower in ClO2-treated embryos. We found no gross or microscopic evidence of toxicity caused by ClO2 at the doses used herein.

Conclusions Our study shows that ClO2 could be a safe and viable way of treating and mitigating the effects of avian coronavirus infections, and raises the possibility that similar effects could be observed in other organisms.

Figure
  • Download figure
  • Open in new tab

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • We have modified the manuscript slightly as well as the supplementary materials to remove a statistical approach that was not offering additional information to the manuscript and that could be misleading.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted November 10, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Evaluation of the antiviral effect of chlorine dioxide (ClO2) using a vertebrate model inoculated with avian coronavirus
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Evaluation of the antiviral effect of chlorine dioxide (ClO2) using a vertebrate model inoculated with avian coronavirus
Xóchitl Zambrano-Estrada, Carlos Domínguez-Sánchez, Marina Banuet-Martínez, Fabiola Guerrero de la Rosa, Teresa García-Gasca, Luis Prieto-Valiente, Karina Acevedo-Whitehouse
bioRxiv 2020.10.13.336768; doi: https://doi.org/10.1101/2020.10.13.336768
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Evaluation of the antiviral effect of chlorine dioxide (ClO2) using a vertebrate model inoculated with avian coronavirus
Xóchitl Zambrano-Estrada, Carlos Domínguez-Sánchez, Marina Banuet-Martínez, Fabiola Guerrero de la Rosa, Teresa García-Gasca, Luis Prieto-Valiente, Karina Acevedo-Whitehouse
bioRxiv 2020.10.13.336768; doi: https://doi.org/10.1101/2020.10.13.336768

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3609)
  • Biochemistry (7590)
  • Bioengineering (5533)
  • Bioinformatics (20833)
  • Biophysics (10347)
  • Cancer Biology (7998)
  • Cell Biology (11662)
  • Clinical Trials (138)
  • Developmental Biology (6619)
  • Ecology (10227)
  • Epidemiology (2065)
  • Evolutionary Biology (13647)
  • Genetics (9557)
  • Genomics (12859)
  • Immunology (7931)
  • Microbiology (19575)
  • Molecular Biology (7678)
  • Neuroscience (42192)
  • Paleontology (309)
  • Pathology (1259)
  • Pharmacology and Toxicology (2208)
  • Physiology (3272)
  • Plant Biology (7064)
  • Scientific Communication and Education (1295)
  • Synthetic Biology (1953)
  • Systems Biology (5434)
  • Zoology (1119)