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Higher temperatures worsen the effects of mutations on protein stability

View ORCID ProfileDimitrios - Georgios Kontopoulos, View ORCID ProfileIlias Patmanidis, View ORCID ProfileTimothy G. Barraclough, View ORCID ProfileSamraat Pawar
doi: https://doi.org/10.1101/2020.10.13.337972
Dimitrios - Georgios Kontopoulos
1Science and Solutions for a Changing Planet DTP, Imperial College London, London, UK
2Department of Life Sciences, Imperial College London, Silwood Park, Ascot, Berkshire, UK
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  • For correspondence: dgkontopoulos@gmail.com
Ilias Patmanidis
3Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, the Netherlands
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Timothy G. Barraclough
2Department of Life Sciences, Imperial College London, Silwood Park, Ascot, Berkshire, UK
4Department of Zoology, University of Oxford, Oxford, Oxfordshire, UK
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Samraat Pawar
2Department of Life Sciences, Imperial College London, Silwood Park, Ascot, Berkshire, UK
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Abstract

Understanding whether and how temperature increases alter the effects of mutations on protein stability is crucial for understanding the limits to thermal adaptation by organisms. Currently, it is generally assumed that the stability effects of mutations are independent of temperature. Yet, mutations should become increasingly destabilizing as temperature rises due to the increase in the energy of atoms. Here, by performing an extensive computational analysis on the essential enzyme adenylate kinase in prokaryotes, we show, for the first time, that mutations become more destabilizing with temperature both across and within species. Consistent with these findings, we find that substitution rates of prokaryotes decrease nonlinearly with temperature. Our results suggest that life on Earth likely originated in a moderately thermophilic and thermally fluctuating environment, and indicate that global warming should decrease the per-generation rate of molecular evolution of prokaryotes.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://doi.org/10.6084/m9.figshare.12635837.v1

  • https://github.com/dgkontopoulos/Kontopoulos_et_al_mutations_vs_temperature_2020

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted October 14, 2020.
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Higher temperatures worsen the effects of mutations on protein stability
Dimitrios - Georgios Kontopoulos, Ilias Patmanidis, Timothy G. Barraclough, Samraat Pawar
bioRxiv 2020.10.13.337972; doi: https://doi.org/10.1101/2020.10.13.337972
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Higher temperatures worsen the effects of mutations on protein stability
Dimitrios - Georgios Kontopoulos, Ilias Patmanidis, Timothy G. Barraclough, Samraat Pawar
bioRxiv 2020.10.13.337972; doi: https://doi.org/10.1101/2020.10.13.337972

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