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A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells

Yu Bai, Emmanuel Caussinus, Stefano Leo, Fritz Bosshardt, Faina Myachina, Gregor Rot, Mark D. Robinson, View ORCID ProfileChristian F. Lehner
doi: https://doi.org/10.1101/2020.10.15.340596
Yu Bai
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
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Emmanuel Caussinus
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
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Stefano Leo
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
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Fritz Bosshardt
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
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Faina Myachina
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
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Gregor Rot
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
2SIB Swiss Institute of Bioinformatics, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
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Mark D. Robinson
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
2SIB Swiss Institute of Bioinformatics, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
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Christian F. Lehner
1Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
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  • ORCID record for Christian F. Lehner
  • For correspondence: christian.lehner@mls.uzh.ch
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Abstract

Cells of many ectothermic species, including Drosophila melanogaster, maintain homeostatic function within a considerable temperature range. The cellular mechanisms enabling temperature acclimation are still poorly understood. At the transcriptional level, the heat shock response has been extensively analyzed. The opposite has received less attention. Here, using cultured Drosophila cells, we have identified genes with increased transcript levels at the lower end of the readily tolerated temperature range, as well as chromatin regions with increased DNA accessibility. Candidate cis-regulatory elements (CREs) for transcriptional upregulation at low temperature were selected and evaluated with a novel reporter assay for accurate assessment of their temperature-dependency. Robust transcriptional upregulation at low temperature could be demonstrated for a fragment from the pastrel gene, which expresses more transcript and protein at reduced temperatures. The CRE is controlled by the JAK/STAT signaling pathway and antagonizing activities of the transcription factors Pointed and Ets97D.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 15, 2020.
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A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells
Yu Bai, Emmanuel Caussinus, Stefano Leo, Fritz Bosshardt, Faina Myachina, Gregor Rot, Mark D. Robinson, Christian F. Lehner
bioRxiv 2020.10.15.340596; doi: https://doi.org/10.1101/2020.10.15.340596
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A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells
Yu Bai, Emmanuel Caussinus, Stefano Leo, Fritz Bosshardt, Faina Myachina, Gregor Rot, Mark D. Robinson, Christian F. Lehner
bioRxiv 2020.10.15.340596; doi: https://doi.org/10.1101/2020.10.15.340596

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