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Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules

View ORCID ProfileArik Shams, Sean A. Higgins, View ORCID ProfileChristof Fellmann, View ORCID ProfileThomas J. Laughlin, Benjamin L. Oakes, View ORCID ProfileRachel Lew, Maria Lukarska, Madeline Arnold, Brett T. Staahl, View ORCID ProfileJennifer A. Doudna, View ORCID ProfileDavid F. Savage
doi: https://doi.org/10.1101/2020.10.19.344077
Arik Shams
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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Sean A. Higgins
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
3Scribe Therapeutics, Alameda, CA 94501, USA
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Christof Fellmann
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
4Gladstone Institutes, San Francisco, CA 94158, USA
5Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
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  • ORCID record for Christof Fellmann
Thomas J. Laughlin
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
6Division of Biological Sciences, University of San Diego, San Diego, CA 92093, USA
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Benjamin L. Oakes
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
3Scribe Therapeutics, Alameda, CA 94501, USA
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Rachel Lew
4Gladstone Institutes, San Francisco, CA 94158, USA
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  • ORCID record for Rachel Lew
Maria Lukarska
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
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Madeline Arnold
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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Brett T. Staahl
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
3Scribe Therapeutics, Alameda, CA 94501, USA
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Jennifer A. Doudna
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
4Gladstone Institutes, San Francisco, CA 94158, USA
7Graduate Group in Biophysics, University of California, Berkeley, Berkeley, CA 94720, USA
8Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA
9Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
10Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
11Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA
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  • ORCID record for Jennifer A. Doudna
David F. Savage
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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  • ORCID record for David F. Savage
  • For correspondence: savage@berkeley.edu
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Article Information

doi 
https://doi.org/10.1101/2020.10.19.344077
History 
  • October 19, 2020.

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  • You are currently viewing Version 1 of this article (October 19, 2020 - 19:02).
  • View Version 2, the most recent version of this article.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Arik Shams1,*,
  2. Sean A. Higgins1,2,3,*,
  3. Christof Fellmann1,4,5,
  4. Thomas J. Laughlin1,6,
  5. Benjamin L. Oakes1,2,3,
  6. Rachel Lew4,
  7. Maria Lukarska1,2,
  8. Madeline Arnold1,
  9. Brett T. Staahl1,2,3,
  10. Jennifer A. Doudna1,2,4,7,8,9,10,11 and
  11. David F. Savage1,^
  1. 1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
  2. 2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
  3. 3Scribe Therapeutics, Alameda, CA 94501, USA
  4. 4Gladstone Institutes, San Francisco, CA 94158, USA
  5. 5Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
  6. 6Division of Biological Sciences, University of San Diego, San Diego, CA 92093, USA
  7. 7Graduate Group in Biophysics, University of California, Berkeley, Berkeley, CA 94720, USA
  8. 8Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA
  9. 9Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
  10. 10Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
  11. 11Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA
  1. ↵^Correspondence: savage{at}berkeley.edu.
  1. ↵* These authors contributed equally to this work.

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Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
Arik Shams, Sean A. Higgins, Christof Fellmann, Thomas J. Laughlin, Benjamin L. Oakes, Rachel Lew, Maria Lukarska, Madeline Arnold, Brett T. Staahl, Jennifer A. Doudna, David F. Savage
bioRxiv 2020.10.19.344077; doi: https://doi.org/10.1101/2020.10.19.344077
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Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
Arik Shams, Sean A. Higgins, Christof Fellmann, Thomas J. Laughlin, Benjamin L. Oakes, Rachel Lew, Maria Lukarska, Madeline Arnold, Brett T. Staahl, Jennifer A. Doudna, David F. Savage
bioRxiv 2020.10.19.344077; doi: https://doi.org/10.1101/2020.10.19.344077

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