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Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules

View ORCID ProfileArik Shams, Sean A. Higgins, View ORCID ProfileChristof Fellmann, View ORCID ProfileThomas J. Laughlin, Benjamin L. Oakes, View ORCID ProfileRachel Lew, Maria Lukarska, Madeline Arnold, Brett T. Staahl, View ORCID ProfileJennifer A. Doudna, View ORCID ProfileDavid F. Savage
doi: https://doi.org/10.1101/2020.10.19.344077
Arik Shams
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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  • ORCID record for Arik Shams
Sean A. Higgins
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
3Scribe Therapeutics, Alameda, CA 94501, USA
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Christof Fellmann
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
4Gladstone Institutes, San Francisco, CA 94158, USA
5Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA
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  • ORCID record for Christof Fellmann
Thomas J. Laughlin
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
6Division of Biological Sciences, University of San Diego, San Diego, CA 92093, USA
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  • ORCID record for Thomas J. Laughlin
Benjamin L. Oakes
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
3Scribe Therapeutics, Alameda, CA 94501, USA
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Rachel Lew
4Gladstone Institutes, San Francisco, CA 94158, USA
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  • ORCID record for Rachel Lew
Maria Lukarska
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
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Madeline Arnold
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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Brett T. Staahl
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
3Scribe Therapeutics, Alameda, CA 94501, USA
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Jennifer A. Doudna
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
2Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA
4Gladstone Institutes, San Francisco, CA 94158, USA
7Graduate Group in Biophysics, University of California, Berkeley, Berkeley, CA 94720, USA
8Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA
9Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
10Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
11Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA
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  • ORCID record for Jennifer A. Doudna
David F. Savage
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
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  • ORCID record for David F. Savage
  • For correspondence: savage@berkeley.edu
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Posted October 19, 2020.
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Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
Arik Shams, Sean A. Higgins, Christof Fellmann, Thomas J. Laughlin, Benjamin L. Oakes, Rachel Lew, Maria Lukarska, Madeline Arnold, Brett T. Staahl, Jennifer A. Doudna, David F. Savage
bioRxiv 2020.10.19.344077; doi: https://doi.org/10.1101/2020.10.19.344077
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Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
Arik Shams, Sean A. Higgins, Christof Fellmann, Thomas J. Laughlin, Benjamin L. Oakes, Rachel Lew, Maria Lukarska, Madeline Arnold, Brett T. Staahl, Jennifer A. Doudna, David F. Savage
bioRxiv 2020.10.19.344077; doi: https://doi.org/10.1101/2020.10.19.344077

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