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Inverse oculomotor responses of achiasmatic mice expressing a transfer-defective Vax1 mutant

Kwang Wook Min, Namsuk Kim, Jae Hoon Lee, Younghoon Sung, Museong Kim, Eun Jung Lee, Jae-Hyun Kim, Jaeyoung Lee, Jong-Myeong Kim, Jee Myung Yang, Seung-Hee Lee, View ORCID ProfileHan-Woong Lee, View ORCID ProfileJin Woo Kim
doi: https://doi.org/10.1101/2020.10.20.346551
Kwang Wook Min
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
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Namsuk Kim
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
2Neurovascular Unit, Korea Brain Research Institute (KBRI), Daegu, South Korea
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Jae Hoon Lee
3Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea
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Younghoon Sung
4Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul, South Korea
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Museong Kim
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
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Eun Jung Lee
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
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Jae-Hyun Kim
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
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Jaeyoung Lee
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
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Jong-Myeong Kim
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
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Jee Myung Yang
5Department of Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea
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Seung-Hee Lee
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
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Han-Woong Lee
3Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea
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  • ORCID record for Han-Woong Lee
Jin Woo Kim
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea
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  • ORCID record for Jin Woo Kim
  • For correspondence: jinwookim@kaist.ac.kr
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ABSTRACT

In animals that exhibit stereoscopic visual responses, the axons of retinal ganglion cells (RGCs) connect to brain areas bilaterally by forming a commissure called the optic chiasm (OC). Ventral anterior homeobox 1 (Vax1) contributes to formation of the OC, acting endogenously in optic pathway cells and exogenously in growing RGC axons. Here, we generated Vax1AA/AA mice expressing the Vax1AA mutant, which is selectively incapable of intercellular transfer. We found that RGC axons cannot take up Vax1AA protein from Vax1AA/AA mouse optic stalk (OS) cells, of which maturation is delayed, and fail to access the midline. Consequently, RGC axons of Vax1AA/AA mice connect exclusively to ipsilateral brain areas, resulting in the loss of stereoscopic vision and the inversed oculomotor responses. Together, our study provides physiological evidence for the necessity of intercellular transfer of Vax1 and the importance of the OC in binocular visual responses.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted October 20, 2020.
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Inverse oculomotor responses of achiasmatic mice expressing a transfer-defective Vax1 mutant
Kwang Wook Min, Namsuk Kim, Jae Hoon Lee, Younghoon Sung, Museong Kim, Eun Jung Lee, Jae-Hyun Kim, Jaeyoung Lee, Jong-Myeong Kim, Jee Myung Yang, Seung-Hee Lee, Han-Woong Lee, Jin Woo Kim
bioRxiv 2020.10.20.346551; doi: https://doi.org/10.1101/2020.10.20.346551
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Inverse oculomotor responses of achiasmatic mice expressing a transfer-defective Vax1 mutant
Kwang Wook Min, Namsuk Kim, Jae Hoon Lee, Younghoon Sung, Museong Kim, Eun Jung Lee, Jae-Hyun Kim, Jaeyoung Lee, Jong-Myeong Kim, Jee Myung Yang, Seung-Hee Lee, Han-Woong Lee, Jin Woo Kim
bioRxiv 2020.10.20.346551; doi: https://doi.org/10.1101/2020.10.20.346551

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