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SARS-CoV-2 specific memory T lymphocytes from COVID-19 convalescent donors: identification, biobanking and large-scale production for Adoptive Cell Therapy

C Ferreras, B Pascual-Miguel, C Mestre-Durán, A Navarro-Zapata, L Clares-Villa, C Martín-Cortázar, R De Paz, A Marcos, JL Vicario, A Balas, F García-Sánchez, C Eguizabal, C Solano, M Mora-Rillo, B Soria, A Pérez-Martínez
doi: https://doi.org/10.1101/2020.10.23.352294
C Ferreras
1Hospital La Paz Institute for Health Research, IdiPAZ, La Paz University Hospital, Madrid, Spain
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B Pascual-Miguel
1Hospital La Paz Institute for Health Research, IdiPAZ, La Paz University Hospital, Madrid, Spain
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C Mestre-Durán
1Hospital La Paz Institute for Health Research, IdiPAZ, La Paz University Hospital, Madrid, Spain
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A Navarro-Zapata
1Hospital La Paz Institute for Health Research, IdiPAZ, La Paz University Hospital, Madrid, Spain
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L Clares-Villa
1Hospital La Paz Institute for Health Research, IdiPAZ, La Paz University Hospital, Madrid, Spain
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C Martín-Cortázar
1Hospital La Paz Institute for Health Research, IdiPAZ, La Paz University Hospital, Madrid, Spain
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R De Paz
2University Hospital La Paz, Hematology Department, Madrid, Spain
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A Marcos
2University Hospital La Paz, Hematology Department, Madrid, Spain
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JL Vicario
3Histocompatibility. Centro de Transfusión de Madrid. Madrid, Spain
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A Balas
3Histocompatibility. Centro de Transfusión de Madrid. Madrid, Spain
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F García-Sánchez
3Histocompatibility. Centro de Transfusión de Madrid. Madrid, Spain
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C Eguizabal
4Research Unit, Basque Center for Blood Transfusion and Human Tissues, Osakidetza, Galdakao, Bizkaia, Spain
5Cell Therapy, Stem Cells and Tissues Group, Biocruces Bizkaia Health Research Institute, Barakaldo, Bizkaia, Spain
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C Solano
6Hospital Clínico Universitario de Valencia/Instituto de Investigación Sanitaria INCLIVA, Universidad de Valencia
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M Mora-Rillo
7Infectious Diseases Unit, Internal Medicine Department, University Hospital La Paz, - Hospital La Paz Institute for Health Research, IdiPAZ, La Paz University Hospital, Madrid, Spain
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B Soria
8Instituto de Bioingeniería, Universidad Miguel Hernández de Elche, Alicante
9Instituto de Investigación Sanitaria Hospital General y Universitario de Alicante ISABIAL
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A Pérez-Martínez
1Hospital La Paz Institute for Health Research, IdiPAZ, La Paz University Hospital, Madrid, Spain
10University Hospital La Paz, Pediatric Hemato-oncology Department, Madrid, Spain
11Faculty of Medicine Universidad Autónoma de Madrid, Madrid, Spain
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  • For correspondence: aperezmartinez@salud.madrid.org
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ABSTRACT

SARS-CoV-2 is causing a second outbreak so the hope for its complete eradication is far from happening. In the absence of effective vaccines, it is mandatory to find effective treatments with low adverse effects able to treat hospitalized patients with COVID-19 disease. In this work, we determined the existence of SARS-CoV-2 specific T cells within the CD45RA− T memory cells from the blood of convalescent donors. Memory T cells can respond quickly to the infection and provide long-term immune protection to reduce the severity of the COVID-19 symptoms. Also, CD45RA− memory T cells confer protection from other pathogens the donors encountered in their life. This is vital to clear other secondary infections usually developed in hospitalized COVID-19 patients. SARS-CoV-2 specific memory T cells were found within all the CD45RA− subsets CD3+, CD4+, CD8+, and in the central memory and effector memory subpopulations. The procedure to obtain the cells is feasible, easy to implement for small scale manufacture, quick and cost-effective involving minimal manipulation, and without GMP condition requirements. This biobank of specific SARS-CoV-2 memory T cells would be immediately available ‘off-the-shelf’ to treat moderate/severe cases of COVID-19 increasing the therapeutic options available for these patients.

Competing Interest Statement

CF, BS and AP-M filed a patent on this topic. BS received fees from Celgene, Gilead, Sanofi and Novo-Nordisk not related to this work.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 26, 2020.
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SARS-CoV-2 specific memory T lymphocytes from COVID-19 convalescent donors: identification, biobanking and large-scale production for Adoptive Cell Therapy
C Ferreras, B Pascual-Miguel, C Mestre-Durán, A Navarro-Zapata, L Clares-Villa, C Martín-Cortázar, R De Paz, A Marcos, JL Vicario, A Balas, F García-Sánchez, C Eguizabal, C Solano, M Mora-Rillo, B Soria, A Pérez-Martínez
bioRxiv 2020.10.23.352294; doi: https://doi.org/10.1101/2020.10.23.352294
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SARS-CoV-2 specific memory T lymphocytes from COVID-19 convalescent donors: identification, biobanking and large-scale production for Adoptive Cell Therapy
C Ferreras, B Pascual-Miguel, C Mestre-Durán, A Navarro-Zapata, L Clares-Villa, C Martín-Cortázar, R De Paz, A Marcos, JL Vicario, A Balas, F García-Sánchez, C Eguizabal, C Solano, M Mora-Rillo, B Soria, A Pérez-Martínez
bioRxiv 2020.10.23.352294; doi: https://doi.org/10.1101/2020.10.23.352294

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