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Regulation of touch dependant de novo root regeneration in Arabidopsis

Anju Pallipurath Shanmukhan, Mabel Maria Mathew, Mohammed Aiyaz, View ORCID ProfileAbdul Kareem, Dhanya Radhakrishnan, View ORCID ProfileKalika Prasad
doi: https://doi.org/10.1101/2020.11.04.367714
Anju Pallipurath Shanmukhan
1School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695551, India
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Mabel Maria Mathew
1School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695551, India
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Mohammed Aiyaz
1School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695551, India
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Abdul Kareem
2School of Life and Environmental Sciences, The University of Sydney, NSW, Australia
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Dhanya Radhakrishnan
1School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695551, India
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Kalika Prasad
1School of Biology, Indian Institute of Science Education and Research, Thiruvananthapuram, Kerala 695551, India
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  • ORCID record for Kalika Prasad
  • For correspondence: kalika@iisertvm.ac.in
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Abstract

The versatile regeneration capability of leaves enable even a detached Arabidopsis leaf to yield two kinds of regenerative responses namely, wound healing at the cut end in form of callus formation or de novo root regeneration (DNRR). Using various experimental approaches, we show that the factor favouring DNRR over callus formation seems to be a mechanical cue, possibly touch, at the cut end of the detached leaf. Here, we show that the forced expression of a PLETHORA transcription factor can bypass the need for touch to initiate DNRR. Our findings provide a genetic frame-work for touch dependant DNRR and suggest that a core PLT transcription regulatory module acts in response to mechano-sensing stimuli.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted November 05, 2020.
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Regulation of touch dependant de novo root regeneration in Arabidopsis
Anju Pallipurath Shanmukhan, Mabel Maria Mathew, Mohammed Aiyaz, Abdul Kareem, Dhanya Radhakrishnan, Kalika Prasad
bioRxiv 2020.11.04.367714; doi: https://doi.org/10.1101/2020.11.04.367714
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Regulation of touch dependant de novo root regeneration in Arabidopsis
Anju Pallipurath Shanmukhan, Mabel Maria Mathew, Mohammed Aiyaz, Abdul Kareem, Dhanya Radhakrishnan, Kalika Prasad
bioRxiv 2020.11.04.367714; doi: https://doi.org/10.1101/2020.11.04.367714

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