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Designer Scaffolds for Bioengineered Tissue Interfaces

Ryan J. Hickey, Maxime Leblanc Latour, View ORCID ProfileJames L. Harden, View ORCID ProfileAndrew E. Pelling
doi: https://doi.org/10.1101/2020.11.06.371278
Ryan J. Hickey
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt, University of Ottawa, Ottawa, ON, K1N6N5 Canada
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Maxime Leblanc Latour
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt, University of Ottawa, Ottawa, ON, K1N6N5 Canada
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James L. Harden
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt, University of Ottawa, Ottawa, ON, K1N6N5 Canada
2Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, K1H8M5 Canada
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Andrew E. Pelling
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt, University of Ottawa, Ottawa, ON, K1N6N5 Canada
3Department of Biology, Gendron Hall, 30 Marie Curie, University of Ottawa, Ottawa, ON, K1N5N5 Canada
4Institute for Science Society and Policy, Simard Hall, 60 University, University of Ottawa, Ottawa, ON, K1N5N5 Canada
5SymbioticA, School of Anatomy, Physiology and Human Biology, University of Western Australia, Perth, WA, 6009
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  • For correspondence: a@pellinglab.net
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ABSTRACT

In regenerative medicine, the healing of the interfacial zone between tissues is a major challenge, yet approaches for engineering and studying the complex microenvironment of this interface remain lacking1–3. Here, we create and study these complex living interfaces by manufacturing modular “blocks” of decellularized plant-derived scaffolds4–6 with varying shapes and sizes with a computer numerical controlled mill. Each block can then be seeded with different cell types and easily assembled in a manner akin to LEGO™ bricks to create an engineered tissue interface (ETI). As a proof-of-concept study we utilize ETIs to investigate the interaction between lab grown bone and connective tissues. We also demonstrate how ETIs are biocompatible in vivo, stimulating the formation of blood vessels, cell infiltration, and tissue integration after implantation. This work creates possibilities for new tissue design avenues for understanding fundamental biological processes or the development of synthetic artificial tissues.

Competing Interest Statement

RJH oversaw all experimental protocols and fabricated the scaffolds, performed cell culture, microscopy and analysis. MLL performed cell culture. MLL and RJH performed the surgical procedures. RJH and JLH performed rheological analyses. AEP conceived, directed and managed the project. All authors reviewed the data and prepared the manuscript for publication.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted June 14, 2021.
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Designer Scaffolds for Bioengineered Tissue Interfaces
Ryan J. Hickey, Maxime Leblanc Latour, James L. Harden, Andrew E. Pelling
bioRxiv 2020.11.06.371278; doi: https://doi.org/10.1101/2020.11.06.371278
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Designer Scaffolds for Bioengineered Tissue Interfaces
Ryan J. Hickey, Maxime Leblanc Latour, James L. Harden, Andrew E. Pelling
bioRxiv 2020.11.06.371278; doi: https://doi.org/10.1101/2020.11.06.371278

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