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Chemoreceptor Co-Expression in Drosophila Olfactory Neurons

View ORCID ProfileDarya Task, View ORCID ProfileChun-Chieh Lin, View ORCID ProfileAlina Vulpe, View ORCID ProfileAli Afify, View ORCID ProfileSydney Ballou, View ORCID ProfileMaria Brbić, View ORCID ProfilePhilipp Schlegel, View ORCID ProfileGregory S. X. E. Jefferis, Hongjie Li, View ORCID ProfileKaren Menuz, View ORCID ProfileChristopher J. Potter
doi: https://doi.org/10.1101/2020.11.07.355651
Darya Task
1The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
10Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA
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Chun-Chieh Lin
1The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
2Mortimer B. Zuckermann Mind Brain Behavior Institute, Columbia University, New York, NY 10027, USA
7The Department of Pathology and Laboratory Medicine, Giesel School of Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756, USA
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Alina Vulpe
3Physiology & Neurobiology Department, University of Connecticut, Mansfield, CT 06269, USA
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Ali Afify
1The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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Sydney Ballou
3Physiology & Neurobiology Department, University of Connecticut, Mansfield, CT 06269, USA
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Maria Brbić
4Department of Computer Science, Stanford University, Stanford, CA 94305, USA
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Philipp Schlegel
5Drosophila Connectomics Group, Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK
8Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 OQH, UK
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Gregory S. X. E. Jefferis
5Drosophila Connectomics Group, Department of Zoology, University of Cambridge, Cambridge, CB2 3EJ, UK
8Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, CB2 OQH, UK
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Hongjie Li
6Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
9Huffington Center on Aging, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
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Karen Menuz
3Physiology & Neurobiology Department, University of Connecticut, Mansfield, CT 06269, USA
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Christopher J. Potter
1The Solomon H. Snyder Department of Neuroscience, Center for Sensory Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
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  • For correspondence: cpotter@jhmi.edu
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Abstract

Drosophila melanogaster olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main olfactory receptor gene families in Drosophila, the Odorant Receptors (ORs) and the Ionotropic Receptors (IRs). The dozens of odorant binding receptors in each family require at least one co-receptor gene in order to function: Orco for ORs, and Ir25a, Ir8a, and Ir76b for IRs. Using a new genetic knock-in strategy, we targeted the four co-receptors representing the main chemosensory families in Drosophila (Orco, Ir8a, Ir76b, Ir25a). Co-receptor knock-in expression patterns were verified as accurate representations of endogenous expression. We find extensive overlap in expression among the different co-receptors. As defined by innervation into antennal lobe glomeruli, Ir25a is broadly expressed in 88% of all olfactory sensory neuron classes and is co-expressed in 82% of Orco+ neuron classes, including all neuron classes in the maxillary palp. Orco, Ir8a, and Ir76b expression patterns are also more expansive than previously assumed. Single sensillum recordings from Orco-expressing Ir25a mutant antennal and palpal neurons identify changes in olfactory responses. These results suggest co-expression of chemosensory receptors is common in olfactory neurons. Together, our data present the first comprehensive map of chemosensory co-receptor expression and reveal their unexpected widespread co-expression in the fly olfactory system.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted July 27, 2021.
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Chemoreceptor Co-Expression in Drosophila Olfactory Neurons
Darya Task, Chun-Chieh Lin, Alina Vulpe, Ali Afify, Sydney Ballou, Maria Brbić, Philipp Schlegel, Gregory S. X. E. Jefferis, Hongjie Li, Karen Menuz, Christopher J. Potter
bioRxiv 2020.11.07.355651; doi: https://doi.org/10.1101/2020.11.07.355651
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Chemoreceptor Co-Expression in Drosophila Olfactory Neurons
Darya Task, Chun-Chieh Lin, Alina Vulpe, Ali Afify, Sydney Ballou, Maria Brbić, Philipp Schlegel, Gregory S. X. E. Jefferis, Hongjie Li, Karen Menuz, Christopher J. Potter
bioRxiv 2020.11.07.355651; doi: https://doi.org/10.1101/2020.11.07.355651

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